Polysaccharides of Atractylodes macrocephala Koidz alleviate bursa of Fabricius involution in Magang geese by inhibiting the Wnt signaling pathway

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-05-01 DOI:10.1016/j.psj.2025.105227

Weijun Liang , Zhuokun Liu , Peixi Xie , Luyi Cen , Xiang Zhou , Longsheng Hong , Shuying Gong , Wanyan Li , Nan Cao , Yunbo Tian , Danning Xu , Bingxin Li

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Abstract

Involution of the bursa of Fabricius (BF) with increasing age in avian species leads to decreases in lymphocyte counts and pathogen recognition, thereby compromising immune function and increasing susceptibility to infections. Polysaccharides of Atractylodes macrocephala Koidz (PAMK), a major component of the traditional Chinese medicine Atractylodes macrocephala, increase immunity. This study aimed to explore the impact of PAMK on the BF during involution in Magang geese. Following the prefeeding period, 54 four-month-old Magang geese were selected for the experiment. These geese were evenly divided into three groups. Each group consisted of three replicates, with six geese per replicate. One group was immediately euthanized to collect serum and BF samples. The remaining 36 geese were further divided into two groups: one group was maintained on a standard diet, and the other group was provided a diet supplemented with 400 mg/kg PAMK. One month later, serum and BF samples were collected for analysis. Morphological, organ index and histological observations indicate that PAMK can alleviate the significant reduction in the organ index of the BF during the involution process in Magang geese and mitigate the disorder of follicular arrangement and the decrease in the ratio of the cortical area to the medullary area in the BF. Immunoglobulin and oxidative stress results revealed that PAMK can increase the levels of immunoglobulins and reduce oxidative stress in involuting Magang geese. Functional enrichment analysis revealed that the Wnt signaling pathway plays a significant role in alleviating the involution of the BF in Magang geese. Further cellular studies revealed that the addition of PAMK inhibited apoptosis and promoted cell cycle progression into the S and G2 phases. In summary, PAMK effectively mitigates the involution of the BF in Magang geese by inhibiting the Wnt signaling pathway, thereby enhancing humoral immunity and improving antioxidant status.

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白术多糖通过抑制Wnt信号通路减轻马钢鹅法氏囊退化

随着年龄的增长，鸟类法氏囊（BF）的内化导致淋巴细胞计数和病原体识别减少，从而损害免疫功能并增加对感染的易感性。白术多糖（PAMK）是中药苍术的主要成分，具有增强免疫力的作用。本试验旨在探讨PAMK对马岗鹅退化期BF的影响。预饲期结束后，选取54只4月龄马岗鹅进行试验。这些鹅被平均分成三组。每组设3个重复，每个重复6只鹅。一组立即安乐死，采集血清和BF样本。其余36只鹅分为2组，1组饲喂标准饲粮，1组饲喂在标准饲粮中添加400 mg/kg PAMK的饲粮。1个月后，采集血清和BF样本进行分析。形态学、器官指数和组织学观察结果表明，PAMK能缓解马岗鹅蛋形复归过程中蛋形器官指数的显著下降，减轻蛋形卵泡排列紊乱和蛋形皮质面积/髓质面积比值的降低。免疫球蛋白和氧化应激结果表明，PAMK可以提高免疫球蛋白水平，降低氧化应激。功能富集分析表明，Wnt信号通路在缓解马岗鹅BF退化中发挥了重要作用。进一步的细胞研究表明，PAMK的加入抑制了细胞凋亡，促进了细胞周期进入S期和G2期。综上所述，PAMK通过抑制Wnt信号通路，有效减轻马钢鹅BF的退化，从而增强体液免疫，改善抗氧化状态。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.