Chicken IL-17 as a potent adjuvant enhances IBDV VP2 DNA vaccine immunogenicity and protective efficacy

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-05-01 DOI:10.1016/j.psj.2025.105247

Jianlou Zhang , Wenyan Li , Shanshan Huo , Xiaojing Xin , Liyue Wang

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引用次数: 0

Abstract

Infectious bursal disease virus (IBDV) is an important immunosuppressive virus in chickens, which caused an acute and highly contagious infectious disease and resulted in considerable economic losses in the world poultry industry. Although IBDV VP2 DNA vaccine has been extensively proved only partial protection, the efficacy of DNA vaccines against IBDV can be augmented by choosing potential molecular adjuvants. To investigate whether chicken IL-17 (chIL-17) is qualified for the molecular adjuvant, we constructed chIL-17 gene vector, co-immunized chickens with chIL-17 gene vector and IBDV VP2 DNA vector, and analyzed the effect of IL-17 gene on the immunogenicity of VP2 DNA vaccine. Results showed that co-administrated chIL-17 gene and VP2 DNA vaccine significantly increased IBDV-specific serum antibody titer, and promoted lymphocyte proliferation and interference-γ (IFN-γ) production. Mechanically, chIL-17 reduced virus titer in bursa of fabricius tissue and mortality of IBDV-infected chicken. More importantly, chIL-17 gene significantly enhanced the protection induced by the VP2 gene against virulent IBDV infection.These data show that chIL-17 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against IBDV, and suggest that it should be evaluated as a new DNA vaccine adjuvant in strategies for other poultry diseases.

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鸡IL-17作为有效佐剂增强了IBDV VP2 DNA疫苗的免疫原性和保护效果

传染性法氏囊病病毒（IBDV）是鸡体内一种重要的免疫抑制病毒，是一种具有高度传染性的急性传染病，给世界家禽业造成了巨大的经济损失。尽管IBDV VP2 DNA疫苗已被广泛证明仅具有部分保护作用，但可以通过选择潜在的分子佐剂来增强DNA疫苗对IBDV的功效。为研究鸡IL-17 （chill -17）是否适合作为分子佐剂，我们构建了chill -17基因载体，用chill -17基因载体和IBDV VP2 DNA载体共免疫鸡，并分析了IL-17基因对VP2 DNA疫苗免疫原性的影响。结果表明，chIL-17基因与VP2 DNA疫苗联合接种可显著提高ibdv特异性血清抗体滴度，促进淋巴细胞增殖和干扰素γ (IFN-γ)的产生。从机械上讲，chIL-17降低了ibdv感染鸡的法氏囊组织中的病毒滴度和死亡率。更重要的是，chIL-17基因显著增强了VP2基因诱导的对IBDV强毒感染的保护作用。这些数据表明，chIL-17是一种很有前景的免疫佐剂，可以促进IBDV疫苗的体液和细胞免疫，并建议将其作为一种新的DNA疫苗佐剂用于其他家禽疾病的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.