Thermostable phospholipase C: A key to efficient and sustainable enzymatic oil degumming processes

IF 1.9 4区农林科学 Q3 CHEMISTRY, APPLIED

Journal of the American Oil Chemists Society Pub Date : 2025-01-24 DOI:10.1002/aocs.12934

Diego S. Val, Luisina Di Nardo, Fiorela Marchisio, Franco Lacava, Andrés Aguirre, Salvador Peiru, María Eugenia Castelli, Martín Espariz, Luciano A. Abriata, Hugo G. Menzella, Rodolfo M. Rasia

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Abstract

Phospholipase C (PLC) enzymes play a pivotal role in the degumming process of edible oils, which is essential for producing high-quality refined oils. The enzymatic degumming process, compared with conventional chemical methods, offers significant advantages including improved oil yield, reduced environmental impact, and lower operational costs. However, the industrial application of PLC enzymes is often hindered by their limited stability under harsh processing conditions. This has driven extensive research efforts toward engineering thermostable PLC variants that can withstand the high temperatures and harsh environments typical of oil refining processes. In this review, we explore the latest advancements in the application, design and optimization of thermostable PLC enzymes. We discuss the fundamental economic and ecological interest behind the goal of obtaining thermostable enzymes, and two approaches to the problem, namely the search for enzymes in thermophilic organisms and the design of new sequences with improved stability and activity parameters.

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耐热磷脂酶C：高效和可持续的酶解油脱胶工艺的关键

磷脂酶C （PLC）酶在食用油脱胶过程中起着关键作用，是生产高品质成品油所必需的。与传统的化学方法相比，酶解脱胶工艺具有显著的优势，包括提高原油产量、减少对环境的影响、降低运营成本。然而，PLC酶的工业应用往往受到其在恶劣加工条件下有限的稳定性的阻碍。这推动了对工程耐热PLC变体的广泛研究，这些变体可以承受炼油过程中典型的高温和恶劣环境。本文综述了耐热型PLC酶在应用、设计和优化方面的最新进展。我们讨论了获得热稳定酶的目标背后的基本经济和生态利益，以及两种解决问题的方法，即在嗜热生物中寻找酶和设计具有更好稳定性和活性参数的新序列。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the American Oil Chemists Society 工程技术-食品科技

CiteScore

4.10

自引率

5.00%

发文量

审稿时长

2.4 months

期刊介绍： The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate. JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.