Isolation and Identification of a Novel Chitinilyticum sp.C8: Unleashing the Potential for Chitin Oligosaccharides Production

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-06 DOI:10.1021/acs.jafc.4c10654

Qiong Zhao, Liqiang Fan, Yanan Cui, Mengxuan Zhao, Chen Deng, Liming Zhao

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Abstract

Chitin oligosaccharides (CHOS) possess potential applications in food, medicine, and agriculture. Nevertheless, their low production efficiency and polymerization degree severely restrict their extensive utilization. In the present study, a Chitinase-producing bacterium was isolated and identified as Chitinilyticum sp.C8. Under the optimized conditions, the Chitinase yield reached 2.24 U/mL. Using extracellular Chitinase derived from Chitinilyticum sp.C8, chitin was converted to CHOS (DP 2–7) and GlcNAc with a yield of 96.7%. Furthermore, the endochitinase ChiC8-2 was isolated from crude enzyme preparation, which can hydrolyze chitin to obtain CHOS (DP 2–6) ((GlcNAc)₂ 0.2 mg/mL (22.7%), (GlcNAc)₃ 0.03 mg/mL (3.4%), (GlcNAc)₄ 0.25 mg/mL (28.4%), (GlcNAc)₅ 0.09 mg/mL (10.2%), and (GlcNAc)₆ 0.21 mg/mL (23.9%)) within 30 min. The Chitinilyticum sp.C8 could not only produce various types of chitinases but also pretreat chitin to improve the efficiency of enzymatic hydrolysis. An integrated preparation method from Chitinase production to chitin treatment by Chitinilyticum sp.C8 was developed for the efficient production of CHOS. This study introduces an environmentally friendly strategy for the production of bioactive CHOS, facilitating the sustainable utilization of renewable natural resources.

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新型几丁质菌c8的分离鉴定：释放几丁质低聚糖生产潜力

几丁寡糖（CHOS）在食品、医药和农业等领域具有潜在的应用前景。但其生产效率低，聚合度低，严重制约了其广泛利用。本研究分离了一种产几丁质酶的细菌，鉴定为几丁质酵母菌c8。在优化条件下，几丁质酶产率达到2.24 U/mL。利用胞外几丁质酶（Chitinilyticum sp.C8）将几丁质转化为CHOS （DP 2-7）和GlcNAc，产率为96.7%。从粗酶制剂中分离到几丁质内切酶ChiC8-2，可在30 min内水解几丁质，得到CHOS (DP 2 - 6) （GlcNAc)2 0.2 mg/mL（22.7%）、（GlcNAc）3 0.03 mg/mL（3.4%）、（GlcNAc）4 0.25 mg/mL（28.4%）、（GlcNAc）5 0.09 mg/mL（10.2%）和（GlcNAc）6 0.21 mg/mL(23.9%)）。c8不仅能产生各种类型的几丁质酶，还能对几丁质进行预处理，提高酶解效率。研究了从几丁质酶生产到利用几丁质菌c8处理几丁质的一体化制备方法，以期高效生产CHOS。本研究介绍了一种生产生物活性有机污染物的环境友好型策略，促进可再生自然资源的可持续利用。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.