A novel idiotypic-based noncompetitive immunoassay based on the dissociation of immunocomplex for detection atrazine in vegetables

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-05-06 DOI:10.1016/j.foodchem.2025.144666

Xing Zhang, Beibei Liu, Rongqi Zhai, Guangyang Liu, Linglong Chen, Junjiang Liu, Maojun Jin, Jing Wang, Donghui Xu, Ge Chen

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Abstract

Atrazine is a selective systemic herbicide of the chlorotriazine class that is commonly used for controlling annual broadleaf and grassy weeds. However, its residue can harm the environment and human health. We have therefore developed a novel idiotypic-based noncompetitive immunoassay for the detection of atrazine by exploiting the distinctive recognition properties of anti-idiotypic antibodies. This method utilizes atrazine as a “key” to specifically dissociation the “lock” of an anti-idiotypic antibody and detection antibody immunocomplex, thereby enabling both qualitative and quantitative analysis of atrazine. Following all necessary optimizations, this method was capable exhibiting a limit of detection (LOD) of 0.52 ng/mL with R² = 0.990, and a linear range of 1.76 to 2604.61 ng/mL. This immunoassay offered its potential towards detection of atrazine in water and 10 kinds of vegetable samples with a recovery ranging from 70.76 to 119.28 %. The idiotypic-based noncompetitive immunoassay provides a well suitable method for low-molecular-weight contaminants immunoassay.

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基于免疫复合物解离的新型独特型非竞争免疫分析法检测蔬菜中莠去津

阿特拉津是氯三嗪类的一种选择性系统除草剂，通常用于控制一年生阔叶杂草和禾本科杂草。但其残留会对环境和人体健康造成危害。因此，我们开发了一种新的基于独特型的非竞争免疫分析法，利用抗独特型抗体的独特识别特性来检测阿特拉津。该方法利用阿特拉津作为“钥匙”特异性解离抗独特型抗体和检测抗体免疫复合物的“锁”，从而实现对阿特拉津的定性和定量分析。经优化，该方法的检出限为0.52 ng/mL, R2 = 0.990，线性范围为1.76 ~ 2604.61 ng/mL。该方法可用于水和10种蔬菜样品中莠去津的检测，回收率为70.76 ~ 119.28 %。基于独特型的非竞争性免疫分析为低分子量污染物的免疫分析提供了一种非常合适的方法。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.