Identification of Δ9 and Δ11 Desaturases Involved in Sex Pheromone Biosynthesis in Mythimna loreyi (Lepidoptera: Noctuidae)

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-01 DOI:10.1021/acs.jafc.5c02139

Liuyang Wang, Pei Guo, Xiaofang Zhang, Yun Duan, Jun Ning, Tao Zhang, Xinling Yang

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Abstract

In moths, sex pheromones are synthesized in pheromone glands (PGs) by a variety of enzymes. Desaturases (DESs) are critical for the introduction of double bonds into pheromones. In Mythimna loreyi, the specific DESs involved in sex pheromone biosynthesis remain unclear. In this study, we identified and characterized 25 putative DESs from the M. loreyi genome. Nineteen of them were expressed in the female PGs, with seven showing significant upregulation in response to pheromone biosynthesis activating neuropeptide (PBAN). RNAi-based knockdown of MlorDES2 significantly reduced the titer of pheromone components by 58.6–85.9%, while knockdown of MlorDES9 specifically reduced the production of (Z)-9-tetradecenyl acetate. Functional verification in yeast revealed that MlorDES2 and MlorDES9 exhibited Δ11 and Δ9 desaturase activities, respectively. Taken together, these results collectively demonstrate that MlorDES2 and MlorDES9 are involved in sex pheromone biosynthesis of M. loreyi, suggesting that DESs could be used as potential targets for pest management.

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夜蛾蛾性信息素合成相关去饱和酶Δ9和Δ11的鉴定（鳞翅目：夜蛾科）

在飞蛾中，性信息素是由多种酶在信息素腺（PGs）中合成的。去饱和酶（DESs）是将双键引入信息素的关键。在神话中，参与性信息素生物合成的特异性DESs尚不清楚。在这项研究中，我们从M. loreyi基因组中鉴定并表征了25个假定的DESs。其中19个基因在雌性PGs中表达，其中7个基因对信息素生物合成激活神经肽（PBAN）表达显著上调。以rnai为基础敲除MlorDES2可显著降低信息素成分滴度58.6-85.9%，而敲除MlorDES9可特异性降低(Z)-9-十四烯乙酸酯的生成。在酵母中的功能验证表明，MlorDES2和MlorDES9分别具有Δ11和Δ9去饱和酶活性。综上所述，这些结果共同表明，MlorDES2和MlorDES9参与了罗氏夜蛾性信息素的生物合成，提示DESs可作为害虫防治的潜在靶点。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.