Interphase cell morphology defines the mode, symmetry, and outcome of mitosis

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2025-05-01

Holly E. Lovegrove, Georgia E. Hulmes, Sabrina Ghadaouia, Christopher Revell, Marta Giralt-Pujol, Zain Alhashem, Andreia Pena, Damian D. Nogare, Ellen Appleton, Guilherme Costa, Richard L. Mort, Christoph Ballestrem, Gareth W. Jones, Cerys S. Manning, Ajay B. Chitnis, Claudio A. Franco, Claudia Linker, Katie Bentley, Shane P. Herbert

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Abstract

During tissue formation, dynamic cell shape changes drive morphogenesis while asymmetric divisions create cellular diversity. We found that the shifts in cell morphology that shape tissues could concomitantly act as conserved instructive cues that trigger asymmetric division and direct core identity decisions underpinning tissue building. We performed single-cell morphometric analyses of endothelial and other mesenchymal-like cells. Distinct morphological changes switched cells to an “isomorphic” mode of division, which preserved pre-mitotic morphology throughout mitosis. In isomorphic divisions, interphase morphology appeared to provide a geometric code defining mitotic symmetry, fate determinant partitioning, and daughter state. Rab4-positive endosomes recognized this code, allowing them to respond to pre-mitotic morphology and segregate determinants accordingly. Thus, morphogenetic shape change sculpts tissue form while also generating cellular heterogeneity, thereby driving tissue assembly.

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间期细胞形态决定有丝分裂的方式、对称性和结果

在组织形成过程中，动态的细胞形态变化驱动形态发生，而不对称分裂创造细胞多样性。我们发现，形成组织的细胞形态的变化可以同时作为保守的指导线索，触发不对称分裂和指导核心身份决定，支持组织构建。我们对内皮细胞和其他间充质样细胞进行了单细胞形态学分析。不同的形态学变化使细胞转变为“同构”分裂模式，这在整个有丝分裂过程中保留了有丝分裂前的形态。在同构分裂中，间期形态似乎提供了定义有丝分裂对称、命运决定分裂和子状态的几何密码。rab4阳性核内体识别这个编码，允许它们对有丝分裂前的形态做出反应，并相应地分离决定因子。因此，形态发生的形状变化塑造了组织形态，同时也产生了细胞异质性，从而推动了组织组装。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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