Field Asymmetric Ion Mobility Spectrometry for Early Detection of Aphanomyces Root Rot in Peas Using Volatile Biomarkers

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI:10.1021/acs.jafc.4c12571

Milton Valencia-Ortiz, Rebecca J. McGee, Sindhuja Sankaran

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引用次数: 0

Abstract

Volatile organic compounds (VOCs) produced by plants during plant–pathogen interactions can be highly informative for early disease detection. The real-time capability of field asymmetric ion mobility spectrometry (FAIMS) offers a valuable opportunity to monitor plant VOCs nondestructively and dynamically. This study evaluated the FAIMS system reliability in measuring VOC profiles for an early diagnosis of Aphanomyces root rot (ARR) in pea (Pisum sativum L.). This evaluation utilized pea lines with a major quantitative trait locus (QTL Ae-Ps7.6) and lines without QTL, identified to provide partial resistance against ARR. For the first time, a VOC biomarker associated with ARR was detected as early as 2 days after inoculation (DAI). Furthermore, at 7 DAI, one of the biomarkers showed significant differences between lines with and without QTL Ae-Ps7.6 in the noninoculated samples. These findings demonstrate the potential applicability of the FAIMS system as a valuable tool for detecting volatile biomarkers for early plant disease detection.

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利用挥发性生物标志物进行豌豆隐匿菌根腐病的田间不对称离子迁移率测定

植物在植物与病原体相互作用过程中产生的挥发性有机化合物（VOCs）可以为早期疾病检测提供大量信息。现场不对称离子迁移谱法（FAIMS）的实时性为植物VOCs的无损动态监测提供了宝贵的机会。本研究评估了FAIMS系统测定挥发性有机化合物谱在豌豆根腐病（apr）早期诊断中的可靠性。本研究利用具有主要数量性状位点（QTL Ae-Ps7.6）的豌豆品系和不具有QTL的豌豆品系对ARR具有部分抗性。首次在接种后2天检测到与ARR相关的VOC生物标志物（DAI）。此外，在第7代，未接种样品中有Ae-Ps7.6 QTL的株系和未接种株系之间的一个生物标记物显示出显著差异。这些发现证明了FAIMS系统作为早期植物病害检测挥发性生物标志物的有价值工具的潜在适用性。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.