Expression and regulation of adrenomedullin 2 gene in ovarian follicles and its effect on follicular granulosa cells in chicken

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-04-22 DOI:10.1016/j.psj.2025.105204

Lihong Li , Dandan Li , Qingqing Wei , Li Kang , Yi Sun , Yunliang Jiang

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引用次数: 0

Abstract

Adrenomedullin 2 (ADM2) is a member of the calcitonin/calcitonin gene-related peptide family and is expressed in various tissues including uterus and ovary. ADM2 is reported to play an important role in ovarian follicle development and steroidogenesis in mammals. In this study, the mRNA expression, the effects of key reproductive hormones on its expression and its effect on follicular granulosa cells were investigated. We found that the expression of ADM2 mRNA was dynamic during follicle development and was lower in the granulosa and theca cells after follicle selection. After treatment with follicle stimulating hormone (FSH), the expression of ADM2 mRNA decreased in the theca cells of pre-hierarchical follicles (Pre-TCs) and hierarchical follicles (Post-TCs), but increased in the granulosa cells of pre-hierarchical follicles (Pre-GCs) and hierarchical follicles (Post-GCs). After treatment with progesterone (P4), the expression of ADM2 mRNA decreased in Pre-TCs, Pre-GCs and Post-GCs, but increased in Post-TCs. After treatment with estradiol (E2), the expression of ADM2 mRNA increased in Post-TCs and Post-GCs. ADM2 suppressed the viability and proliferation of both Pre-GCs and Post-GCs, while facilitated their apoptosis. In both Pre-GCs and Post-GCs, ADM2 promoted the expression of genes involved in P4 synthesis (steroidogenic acute regulatory protein, StAR; cytochrome P450 family 11 subfamily A member 1, CYP11A1; 3β-hydroxysteroid dehydrogenase, 3β-HSD) and P4 secretion. These data suggest that ADM2 plays an important role by decreasing the proliferation and enhancing P4 secretion of granulosa cells around chicken follicle selection.

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鸡卵巢卵泡肾上腺髓质素2基因的表达调控及其对卵泡颗粒细胞的影响

肾上腺髓素2 （ADM2）是降钙素/降钙素基因相关肽家族的成员，在包括子宫和卵巢在内的多种组织中表达。据报道，ADM2在哺乳动物卵巢卵泡发育和类固醇生成中起重要作用。本研究探讨了mRNA的表达、关键生殖激素对其表达的影响及其对卵泡颗粒细胞的影响。我们发现ADM2 mRNA的表达在卵泡发育过程中是动态的，在卵泡选择后的颗粒细胞和卵泡细胞中表达较低。促卵泡激素（FSH）处理后，ADM2 mRNA在分级前卵泡（Pre-TCs）和分级后卵泡（Post-TCs）的膜细胞中表达降低，而在分级前卵泡（Pre-GCs）和分级后卵泡（Post-GCs）的颗粒细胞中表达升高。经黄体酮（P4）治疗后，ADM2 mRNA在tc前、gcs前和gcs后的表达降低，而在tc后的表达升高。经雌二醇（E2）治疗后，ADM2 mRNA在tcs后和gcs后的表达增加。ADM2抑制gcs前和gcs后细胞的活力和增殖，促进其凋亡。在gcs前和gcs后，ADM2均促进了P4合成相关基因的表达(类固醇急性调节蛋白StAR；细胞色素P450家族11亚家族A成员1，CYP11A1；3β-羟基类固醇脱氢酶（3β-HSD）和P4分泌。这些数据表明，ADM2通过降低鸡卵泡选择周围颗粒细胞的增殖和增加P4分泌而发挥重要作用。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.