A phenothiazine-based ratiometric fluorescent probe for detecting hypochlorite (ClO−) and its application in foods and water samples

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-04-26 DOI:10.1016/j.foodchem.2025.144547

Siyi Shen, Yuehua Yuan, Jinping Song, Yongjun Zhu, Yuzhen Wang, Chaoyi Yue, Mengqing Du, Jiyuan Wei, Feng Feng, Maozhong Tian

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引用次数: 0

Abstract

As a significant reactive oxygen species (ROS), ClO⁻ plays versatile roles in daily life and many biological events. However, its abnormal levels are responsible for serious harm to human health. Therefore, it is of crucial interest to develop effective methods for detecting ClO⁻ in foods and living organisms. In this work, a novel fluorescent probe ethyl 2-(3-formyl-2-methoxy-10H-phenothiazin-10-yl)acetate (PEA) for detecting ClO⁻ was presented. It shows the merits of rapid ratiometric response (within 10 s), high selectivity and very large Stokes shift (190 nm). The detection limit of PEA for ClO⁻ was determined to be 0.47 μM. We not only successfully prepared paper test strips for efficient qualitative naked eye ClO⁻ detection, but also demonstrated its potential for the valid detection of ClO⁻ in natural water samples, beverages and foods. Furthermore, the probe PEA was also applied to the fluorescence imaging of ClO⁻ in onion epidermal cells.

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基于吩噻嗪的次氯酸盐（ClO -）比例荧光探针及其在食品和水样中的应用

作为一种重要的活性氧（ROS）， ClO−在日常生活和许多生物事件中发挥着广泛的作用。然而，它的异常水平对人体健康造成严重危害。因此，开发有效的方法来检测食品和生物体内的氯离子是至关重要的。在这项工作中，提出了一种新的荧光探针2-（3-甲酰基-2-甲氧基- 10h -吩噻嗪-10-基）乙酸乙酯（PEA）检测氯离子。它具有快速的比率响应（在10 s内）、高选择性和非常大的Stokes位移（190 nm）的优点。PEA对ClO−的检出限为0.47 μM。我们不仅成功制备了高效定性肉眼检测ClO -的试纸条，而且还证明了其在天然水样、饮料和食品中有效检测ClO -的潜力。此外，还将探针PEA应用于洋葱表皮细胞中ClO−的荧光成像。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.