Construction of a Self-Assembled DNA Nanofirework for Signal Amplification and Intracellular miRNA Imaging

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-04-25 DOI:10.1021/acs.analchem.5c01369

Yuyu Tan, Hongye Mao, Jingping Liu, Hui Chen, Jian Yang, Jiaoli Wang, Jin Huang

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Abstract

Nonenzymatic DNA catalytic amplification strategies have greatly improved the detection of biomolecules. However, the membrane barrier and the complex intracellular environment remain the two main challenges for efficient intracellular RNA imaging. Herein, we designed a self-assembled DNA nanofirework for amplified microRNA (miRNA) imaging in living cells. The self-assembled DNA nanofireworks exhibited high sensitivity and specificity for miRNA detection, achieving excellent internalization efficiency through endocytosis, while demonstrating enhanced biostability and biocompatibility. These properties enable powerful signal-amplified miRNA imaging in live cells. Under optimized conditions, this nanoprobe achieves a linear detection range of 0.5–8.0 nM for miRNA-21 with a detection limit of 89.3 pM. This design represents an optimal integration of DNA nanotechnology with nucleic acid amplification for intracellular biomolecule analysis, providing valuable insights for understanding the biological functions of important biomolecules in disease pathogenesis and potential therapeutic applications.

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用于信号放大和细胞内miRNA成像的自组装DNA纳米烟花的构建

非酶DNA催化扩增策略极大地提高了生物分子的检测。然而，膜屏障和复杂的细胞内环境仍然是有效的细胞内RNA成像的两个主要挑战。在此，我们设计了一个自组装的DNA纳米烟花，用于在活细胞中扩增microRNA （miRNA）成像。自组装DNA纳米烟花对miRNA检测具有很高的灵敏度和特异性，通过内吞作用实现了优异的内化效率，同时具有增强的生物稳定性和生物相容性。这些特性使得在活细胞中进行强大的信号放大miRNA成像成为可能。在优化条件下，该纳米探针对miRNA-21的线性检测范围为0.5 ~ 8.0 nM，检出限为89.3 pM。该设计代表了DNA纳米技术与核酸扩增的最佳整合，用于细胞内生物分子分析，为了解疾病发病机制和潜在治疗应用中重要生物分子的生物学功能提供了有价值的见解。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.