Lipoxygenase 2 is a potential resistance determinant in Brassica juncea – B. fruticulosa derived lines against Lipaphis erysimi infestation

IF 2.4 4区生物学 Q2 PLANT SCIENCES

Acta Physiologiae Plantarum Pub Date : 2025-04-16 DOI:10.1007/s11738-025-03803-7

Jatin Aggarwal, Rimaljeet Kaur, Amandeep Mittal, Chhaya Atri, Mehak Gupta, Anju Sharma

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引用次数: 0

Abstract

Pest infestation on crops trigger high cellular oxidative stress. The strong indicator of oxidative stress initiation are lipoxgenases. We attempted to examine the status of LOX 2 gene and its encoded protein in B. juncea- B. fruticulosa derived lines against Lipaphis erysimi infestation. Cultivated brassicas display lack of resistance against mustard aphid infestation. Brassica fruticulosa, a wild crucifer is a source of enriched resistance gene pool. LOX 2 activity and LOX2 gene expression fold changes were higher in resistant genotypes than susceptible genotypes following 48 h (9.79 and 6.52 fold, respectively) of aphid infestation. Highly resistant test genotype, B. fruticulosa and resistant IL Ad₄-64 displayed 1.87 and 3.83-fold decline in malondialdehyde content at 48 h post-infestation. We depicted pivotal role of LOX 2 in resistance during Brassica-mustard aphid interactions which has not been documented so far.

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脂氧合酶2是甘蓝型芸苔系对褐斑脂肪酶侵染的潜在抗性决定因子

害虫对作物的侵害会引发高细胞氧化应激。脂氧化酶是氧化应激启动的重要指标。本研究旨在研究芥菜b - fruticulosa衍生系抗褐皮虫侵染的lox2基因及其编码蛋白的状态。栽培的芸苔对芥菜蚜缺乏抗性。野生十字花科植物芸苔属（Brassica fruticulosa）具有丰富的抗性基因库。在蚜虫侵染48 h后，抗性基因型的LOX2活性和LOX2基因表达倍数变化均高于敏感基因型（分别为9.79倍和6.52倍）。高抗性试验基因型，果芽孢杆菌和抗性IL Ad4-64侵染后48 h丙二醛含量分别下降1.87倍和3.83倍。我们描述了lox2在油菜-芥菜蚜虫相互作用中抗性的关键作用，迄今为止尚未有文献记载。

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来源期刊

Acta Physiologiae Plantarum 生物-植物科学

CiteScore

5.10

自引率

3.80%

发文量

125

审稿时长

3.1 months

期刊介绍： Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry. The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.