Huddling promotes adult neurogenesis in the brain regions related to social behavior in cold-exposed Brandt’s voles

Abstract

Social thermoregulation behaviors such as huddling among individuals can be important for energy conservation and thermoregulatory processes. Beyond that, whether huddling behavior regulates neural plasticity in the brain remains unknown. We hypothesized that huddling regulates adult neurogenesis in brain regions related to social behavior and thermoregulation. We found that cold-exposed voles had decreased aggression and increased social interaction in association with higher oxytocin but lower vasopressin hormones compared to warm-exposed voles. Cold decreased the level of cell proliferation labeling indicated by BrdU (a marker for cell proliferation) in the anterior part of the subventricular zone (SVZ) in the brain, and notably, cold-huddling (CH) voles had a higher number of proliferating cells in the hypothalamus than cold-separated (CS) voles. Moreover, CH voles displayed higher cell survival in the central amygdala and paraventricular nuclei (PVN) of the hypothalamus (both regions were related to social behavior) in comparison to CS or warm-huddling (WH) voles, respectively. Furthermore, the CH voles had more BrdU/NeuN (markers for new neurons) double-labeled cells in the SVZ than WH voles, and also more BrdU/GFAP (markers for new glial cells) double-labeled cells in SVZ and dentate gyrus compared to WH and CS voles. In addition, the newly-generated neurons differentiated into more oxytocinergic neurons in the PVN of the CH voles. Together, these data support the notion that huddling behavior is beneficial for brain plasticity by protecting cell proliferation, survival, and differentiation, and may be involved in regulating social behavior in small mammals in cold environments.