Rational hapten design, monoclonal antibody preparation, and immunoassays development for rapid detection of fomesafen in agricultural products and soil

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-04-13 DOI:10.1016/j.foodchem.2025.144305

Qingqing Gao , Jingqian Huo , Chang Wang , Jiayu Cao , Haijiao Dong , Jia Kang , Kaiwen Li , Zexiu An , Lai Chen , Jinlin Zhang

{"title":"Rational hapten design, monoclonal antibody preparation, and immunoassays development for rapid detection of fomesafen in agricultural products and soil","authors":"Qingqing Gao , Jingqian Huo , Chang Wang , Jiayu Cao , Haijiao Dong , Jia Kang , Kaiwen Li , Zexiu An , Lai Chen , Jinlin Zhang","doi":"10.1016/j.foodchem.2025.144305","DOIUrl":null,"url":null,"abstract":"<div><div>Fomesafen is a diphenyl ether herbicide, and the residues in soil or agricultural products caused by its misused not only affect the yield and quality of sensitive crops, but also pose a threat to human health through the food chain. In order to achieve rapid and sensitive detection of fomesafen in situ, this study redesigned and synthesized two new haptens based on previous work, and obtained three monoclonal antibodies (mAbs). Indirect competitive ELISA (ic-ELISA) and lateral flow immunoassay (LFIA) methods were established based on heterologous haptens for fomesafen detection. The IC<sub>50</sub> value and linear range for ic-ELISA were 1.56 ± 0.24 ng/mL and 0.08–28.86 ng/mL, respectively. The sensitivity of ic-ELISA developed in this study increased by nearly 25 times compared to our previous work. Meanwhile, the visual limit of detection (vLOD) of LFIA was 2.5 ng/mL with the cutoff value of 16.0 ng/mL. This study is of great significance for achieving rapid on-site detection of fomesafen in agricultural products, soil, and other environmental matrices.</div></div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"483 ","pages":"Article 144305"},"PeriodicalIF":8.5000,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0308814625015560","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Fomesafen is a diphenyl ether herbicide, and the residues in soil or agricultural products caused by its misused not only affect the yield and quality of sensitive crops, but also pose a threat to human health through the food chain. In order to achieve rapid and sensitive detection of fomesafen in situ, this study redesigned and synthesized two new haptens based on previous work, and obtained three monoclonal antibodies (mAbs). Indirect competitive ELISA (ic-ELISA) and lateral flow immunoassay (LFIA) methods were established based on heterologous haptens for fomesafen detection. The IC₅₀ value and linear range for ic-ELISA were 1.56 ± 0.24 ng/mL and 0.08–28.86 ng/mL, respectively. The sensitivity of ic-ELISA developed in this study increased by nearly 25 times compared to our previous work. Meanwhile, the visual limit of detection (vLOD) of LFIA was 2.5 ng/mL with the cutoff value of 16.0 ng/mL. This study is of great significance for achieving rapid on-site detection of fomesafen in agricultural products, soil, and other environmental matrices.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.