Integrated omics reveals the regulatory role of PKCα in Sertoli cell proliferation and apoptosis through the MAPK/ERK signaling pathway in goose testis

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-04-03 DOI:10.1016/j.psj.2025.105123

Yupu Song , Qiuyuan Liu , Jingyun Ma , Ichraf Mabrouk , Yuxuan Zhou , Xinyue Li , Guizhen Xue , Xiaoming Ma , Jing Xu , Jingbo Wang , Hongxiao Pan , Guoqing Hua , Heng Cao , Jingtao Hu , Yongfeng Sun

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引用次数: 0

Abstract

Testicular development is essential for reproductive performance in geese, as the testes are the primary organs for sperm production and play a pivotal role in egg-laying physiology. Despite their importance, genes, proteins, and pathways regulating goose testicular development are poorly understood. This study employed integrative transcriptomic and proteomic analysis methods to identify critical regulators of testicular development in geese across three reproductive periods. Additionally, the role of PKCα in Sertoli cell proliferation via the MAPK/ERK pathway was evaluated at the cellular level. A total of 8,921 differentially expressed genes and 1,866 differentially expressed proteins were identified, revealing key pathways such as FOXO, MAPK, PPAR, and Hedgehog that regulate testicular development. Both omics correlation analysis and signal pathway regulation network results show the importance of MAPK in this process, while cellular experiment revealed that PKCα affects proliferation and apoptosis of Sertoli cells through the MAPK/ERK signaling pathway. The findings revealed that PKCα downregulation reduced the expression of genes associated with both cell proliferation and apoptosis, resulting in a diminished activity of Sertoli cells.

This study compared testicular transcriptomes and proteomes of Hungarian and Jilin white geese, identifying key genes, proteins, and pathways critical for reproduction. These findings advance our understanding of molecular mechanisms underlying testicular development and provide insights to enhance gander reproductive performance.

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.