Irradiation With Cold Atmospheric Direct Plasma: An Innovative Approach to Treating Murine Cutaneous Wounds

IF 2.6 3区物理与天体物理 Q3 ENGINEERING, CHEMICAL

Plasma Chemistry and Plasma Processing Pub Date : 2025-03-01 DOI:10.1007/s11090-025-10555-2

Madyan Ahmed Khalaf, Baida M. Ahmed, Sahar A. H. Al-Sharqi

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Abstract

Cold atmospheric direct plasma (CADP), an ionized gas at ambient temperature, represents a promising approach to enhancing tissue regeneration. A laboratory-based study was conducted to investigate the effects of medical CADP on the reparative potential of full-thickness acute skin wounds in murine models. For the in vivo investigations, two full-thickness dermal injuries were induced in each murine subject, each with a diameter of approximately 8 mm (n = 20). We employed a floating electrode within a CADP system that generates atmospheric pressure air plasma, characterized by a plasma temperature ranging from 36 to 38 °C. The dermal wounds received three plasma treatments, administered every two days, with irradiation durations of 5, 15, and 25 s. These wounds were subsequently evaluated at intervals of 2, 4, 6, 8, and 11 days post-wounding through histological examination and concentration analysis of growth factors. On the eleventh day, the wound healing rates were recorded at 34.80% for the control group, while the plasma-treated groups achieved rates of 56.62%, 84.97%, and 97.82%, respectively. Histological examination revealed that plasma-treatment promotes the development of epidermal keratin and granular strata, along with the formation of hair follicles and sebaceous glands. Concentration analysis of growth factors indicates increased levels of these factors alongside a reduction in white blood cell counts. The CADP therapeutic intervention has significantly enhanced the healing efficacy of acute dermatological lesions without any noticeable adverse effects or the simultaneous activation of pro-inflammatory signaling pathways. These findings highlight the advantages of employing plasma applications for wound management in clinical settings.

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冷大气直接等离子体辐照：治疗小鼠皮肤伤口的创新方法

低温大气直接等离子体（CADP）是一种环境温度下的电离气体，是一种很有前途的增强组织再生的方法。在实验室基础上研究了医用CADP对小鼠全层急性皮肤创伤模型修复潜力的影响。在体内研究中，在每只小鼠受试者中诱导两个全层皮肤损伤，每个直径约为8 mm （n = 20）。我们在CADP系统中使用了一个浮动电极，产生大气压空气等离子体，其特征是等离子体温度范围为36至38°C。皮肤伤口接受三次等离子治疗，每两天给药一次，照射时间分别为5、15和25秒。随后，通过组织学检查和生长因子浓度分析，在伤后2、4、6、8和11天对这些伤口进行评估。第11天，对照组创面愈合率为34.80%，血浆治疗组创面愈合率分别为56.62%、84.97%和97.82%。组织学检查显示，血浆治疗促进表皮角蛋白和颗粒层的发育，并促进毛囊和皮脂腺的形成。生长因子的浓度分析表明，这些因子的水平增加，同时白细胞计数减少。CADP治疗干预显著提高了急性皮肤病病变的愈合效果，无明显的不良反应，也没有同时激活促炎信号通路。这些发现突出了在临床环境中应用等离子体进行伤口管理的优势。

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来源期刊

Plasma Chemistry and Plasma Processing 工程技术-工程：化工

CiteScore

5.90

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Publishing original papers on fundamental and applied research in plasma chemistry and plasma processing, the scope of this journal includes processing plasmas ranging from non-thermal plasmas to thermal plasmas, and fundamental plasma studies as well as studies of specific plasma applications. Such applications include but are not limited to plasma catalysis, environmental processing including treatment of liquids and gases, biological applications of plasmas including plasma medicine and agriculture, surface modification and deposition, powder and nanostructure synthesis, energy applications including plasma combustion and reforming, resource recovery, coupling of plasmas and electrochemistry, and plasma etching. Studies of chemical kinetics in plasmas, and the interactions of plasmas with surfaces are also solicited. It is essential that submissions include substantial consideration of the role of the plasma, for example, the relevant plasma chemistry, plasma physics or plasma–surface interactions; manuscripts that consider solely the properties of materials or substances processed using a plasma are not within the journal’s scope.