Biochemical Studies on the Therapeutic Effect of Naja nubiae Venom Against Melamine Induced Hepatotoxicity in Albino Rats

Abstract

Illegal melamine analogs are added to food to make it appear as though it contains more protein. These substances have negative impacts on both humans and animals in high quantities. The present paper examines how cobra venom shields rats from melamine-induced hepatotoxicity. The current study was conducted on six groups of adult male rats, as follows: group I (negative control): I.P. injected with distilled water, group II (SV10 μg/kg):I.P injected with 10 μg/kg cobra venom, group III (SV20 μg/kg): I.P. injected with 20 μg/kg cobra venom, group IV (melamine): orally 700 mg/kg melamine, group V (melamine + SV10 μg/kg): treated with 10 μg/kg cobra venom, group VI (melamine + SV20 μg/kg): treated with 20 μg/kg cobra venom. Treatment with snake venom ameliorated liver functions and increased apoptotic level marker Caspase-3, decreased anti-apoptotic level marker BAX. Also, decreased inflammatory level marker IL-2 and expression level of IL-10, INF-γ. treatment with snake venom ameliorated hepatotoxicity induced by melamine in albino rats.