Analysis of areca alkaloids and their conversions using CE-C4D and identification by CE-MS

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-04-06 DOI:10.1016/j.foodchem.2025.144228

Jinying Huang , Jiaying Ran , Jingtong Xia , Hongying Du , Lei Zhou

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Abstract

As one of the four major addictive substance globally, areca nut contains areca alkaloids as its principal active ingredients, which exhibit both medicinal potential and toxic effects. In this study, a concise and effective approach was developed for the simultaneous analysis of areca alkaloids using laboratory-built capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C⁴D). The proposed method demonstrated satisfactory linearity (R² ≥ 0.995), precision (RSDs ≤ 6.56%) and quantification limits of 0.77–1.37 μM. This method has been successfully used to quantify areca products with recoveries of 92.2–110.1%. In addition, the effect of processing on the areca alkaloid content in areca nut-containing products was analyzed. On this basis, the conversions of areca alkaloids under various alkaline conditions were investigated through experimental studies and theoretical calculations. The separation parameters for CE-C⁴D were effectively transferred to CE-mass spectrometry (MS), and the above analytical results for areca alkaloids were validated by incorporating CE-MS related experiments.

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CE-C4D法分析槟榔生物碱及其转化，CE-MS法鉴定

槟榔果是全球四大成瘾性物质之一，其主要活性成分为槟榔生物碱，具有药用潜力和毒性作用。本研究建立了一种简便有效的毛细管电泳-电容耦合非接触电导率检测（C4D）同时分析槟榔生物碱的方法。该方法线性良好（R2 ≥ 0.995），精密度（rsd≤6.56 %），定量限为0.77 ~ 1.37 μM。该方法可用于槟榔产品的定量，回收率为92.2 ~ 110.1 %。此外，还分析了加工工艺对槟榔果制品中槟榔生物碱含量的影响。在此基础上，通过实验研究和理论计算，研究了槟榔生物碱在不同碱性条件下的转化。将CE-C4D的分离参数有效转移到CE-MS上，并结合CE-MS相关实验对上述槟榔生物碱的分析结果进行验证。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.