Application of Pyrococcus furiosus Argonaute coupled with RPA for highly sensitive and specific detection of Mycoplasma gallisepticum

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-03-31 DOI:10.1016/j.psj.2025.105116

Yanli Zhao , Dehai Su , Min Li , Qin Deng , Shiyu Xiong , Youshu Feng , Hongxia Jiang , Huanzhong Ding

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引用次数: 0

Abstract

Mycoplasma gallisepticum (MG) is the pathogen that causes chronic respiratory disease (CRD) in chickens and infectious sinusitis in turkeys. Clinical symptoms such as cough and dyspnea cause a decrease in the egg production rate and carcass quality of chickens, which results in considerable economic losses to the poultry industry. Thus, it is urgent to establish a fast, accurate and sensitive method for detecting MG. In this study, the detection of MG by combining recombinase polymerase amplification (RPA) with argonaute from Pyrococcus furiosus (PfAgo) was established that allows visual observation under blue and UV light. The sensitivity of RPA-PfAgo reached 1 copy/µL. After the chicken infection model, it was found that tracheal inhalation was superior to eye and nose drips and sprays, the results of RPA-PfAgo and qPCR were completely consistent. This study provides a reference tool for the monitoring of MG in the clinic and contributes to the early diagnosis of the disease.

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.