Bangle (Zingiber purpureum Rosc.) Extract Ameliorates Colonic Inflammation and Upregulates Autophagy via the Modulation of the AMPK/mTOR/NFκB Pathway in a Mouse Colitis Model

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research Pub Date : 2025-04-03 DOI:10.1002/mnfr.70034

Shiho Kasai, Anishma Karmacharya, Yuuka Mukai, Shin Sato

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Abstract

Bangle, a perennial herb belonging to the ginger family with antiinflammatory properties, has been under-researched in ulcerative colitis. This study aimed to investigate the effects of Bangle extract (BaE) on inflammation and autophagy in the colons of mice with dextran sulfate sodium (DSS)-induced colitis. Male C57BL/6J mice were assigned to four groups: control, DSS + 0% BaE, DSS + 1% BaE, and DSS + 3% BaE. The BaE groups were fed BaE diets for 3 weeks, followed by an additional week of BaE diets and 3% DSS in the water. The control group received a standard chow diet and water for 4 weeks. Plasma leucine-rich α2-glycoprotein (LRG) levels, macrophage count, and the levels of nuclear factor kappa B (NFκB) p65, tumor necrosis factor-α (TNF-α), adenosine monophosphate-activated protein kinase (AMPK), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), mechanistic target of rapamycin (mTOR), and autophagy markers were analyzed. In the DSS + 0% BaE group, LRG levels, macrophage count, NFκB p65 protein, and TNF-α mRNA levels were significantly higher compared to the control group. However, in the DSS + 3% BaE group, these levels were significantly reduced. Additionally, PGC-1α and phosphorylated AMPK levels were increased, while phosphorylated mTOR levels decreased, and autophagy marker microtubule-associated protein 1 light chain 3B (LC3B)-II levels were increased in the DSS + 3% BaE group. BaE may ameliorate colonic inflammation and upregulate autophagy via the modulation of the AMPK/mTOR/NFκB pathway in DSS-induced colitis.

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手镯（Zingiber purpureum Rosc.）在小鼠结肠炎模型中，提取物通过调节AMPK/mTOR/NFκB通路改善结肠炎症并上调自噬

生姜，一种多年生草本植物，属于生姜家族，具有抗炎特性，在溃疡性结肠炎中的研究不足。本研究旨在探讨手乐提取物（BaE）对右旋糖酐硫酸钠（DSS）诱导结肠炎小鼠结肠炎症和自噬的影响。雄性C57BL/6J小鼠分为4组：对照组、DSS + 0% BaE、DSS + 1% BaE和DSS + 3% BaE。各组饲喂BaE饲粮3周，随后在水中添加3% DSS，再添加1周BaE饲粮。对照组给予标准饲料和水喂养4周。分析血浆富白氨酸α2糖蛋白（LRG）水平、巨噬细胞计数、核因子κB (NFκB) p65、肿瘤坏死因子-α (TNF-α)、单磷酸腺苷活化蛋白激酶（AMPK）、过氧化物酶体增殖体活化受体γ共激活因子-1α (PGC-1α)、雷帕霉素机制靶点（mTOR）和自噬标志物水平。DSS + 0% BaE组LRG水平、巨噬细胞计数、nf - κ b p65蛋白、TNF-α mRNA水平均显著高于对照组。然而，在DSS + 3% BaE组，这些水平显著降低。此外，DSS + 3% BaE组PGC-1α和磷酸化AMPK水平升高，磷酸化mTOR水平降低，自噬标志物微管相关蛋白1轻链3B (LC3B)-II水平升高。在dss诱导的结肠炎中，BaE可能通过调节AMPK/mTOR/NFκB通路改善结肠炎症并上调自噬。

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来源期刊

Molecular Nutrition & Food Research 工程技术-食品科技

CiteScore

8.70

自引率

1.90%

发文量

250

审稿时长

1.7 months

期刊介绍： Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.