The Diagnostic Role of TFF1, TFF3, FOXA1, CA XII and TRPS1 in Serous Effusions

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology Pub Date : 2025-04-01 DOI:10.1111/cyt.13489

Ben Davidson, Arild Holth, Jeremias Wohlschlaeger, Maurizio Pinamonti, Binnur Önal

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引用次数: 0

Abstract

Objective

To analyse the diagnostic role of trefoil factor-1 and -3 (TFF1, TFF3), forkhead box protein A1 (FOXA1), carbonic anhydrase XII (CA XII) and trichorhinophalangeal syndrome type 1 (TRPS1) in serous effusions. The prognostic role of these markers in breast carcinoma was additionally studied.

Methods

Protein expression by immunohistochemistry was analysed in 247 effusions, consisting of 60 breast carcinomas, 54 tubo-ovarian carcinomas, 47 mesotheliomas, 44 lung carcinomas, 20 uterine corpus and cervical carcinomas, 17 gastrointestinal carcinomas and 5 cancers of other origin.

Results

TFF1, TFF3, FOXA1, CA XII and TRPS1 expression was found in 67%, 70%, 88%, 82% and 83% of breast carcinomas, respectively. Expression of all markers was seen in some carcinomas of other origin, most commonly in GI metastases, but was least frequent for TRPS1. CA XII expression was additionally seen in mesotheliomas and reactive mesothelial cells. All 5 markers were significantly overexpressed in breast compared to tubo-ovarian carcinoma (all p < 0.001) and lung carcinoma (all p < 0.001 except for FOXA1, p = 0.023). TFF1 (p = 0.003), TFF3 (p = 0.008) and FOXA1 (p = 0.017) expression was significantly higher in receptor-positive compared to receptor-negative primary breast carcinomas. In survival analysis for 44 breast carcinoma patients with clinical data, TFF1 expression was associated with a trend for longer overall (p = 0.096) and disease-free (p = 0.06) survival.

Conclusion

TFF1, TFF3, FOXA1, CA XII and TRPS1 are sensitive breast carcinoma markers, with FOXA1 performing best in terms of sensitivity and TRPS1 being the most specific. Whether the expression of these markers in breast carcinoma effusions is informative of survival merits further research.

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TFF1、TFF3、FOXA1、caxii和TRPS1在浆液积液中的诊断作用

目的分析三叶因子-1和-3（TFF1和TFF3）、叉头盒蛋白A1（FOXA1）、碳酸酐酶XII（CA XII）和毛细血管畸形综合征1型（TRPS1）在浆液性渗出液中的诊断作用。此外，还研究了这些标记物在乳腺癌中的预后作用：方法：对 247 例渗出液进行免疫组织化学分析，其中包括 60 例乳腺癌、54 例输卵管卵巢癌、47 例间皮瘤、44 例肺癌、20 例子宫体癌和宫颈癌、17 例胃肠道癌和 5 例其他来源的癌症：结果发现，67%、70%、88%、82%和83%的乳腺癌中分别有TFF1、TFF3、FOXA1、CA XII和TRPS1的表达。所有标记物的表达均见于一些其他来源的癌症，最常见于消化道转移癌，但TRPS1的表达最少。CA XII还可见于间皮瘤和反应性间皮细胞。与输卵管卵巢癌相比，所有 5 个标记物在乳腺癌中均明显过表达（均为 p 结论：TFF1、TFF3、TFF4、TFF5、TFF6、TFF7、TFF8、TFF9、TFF10、TFF11、TFF12）：TFF1、TFF3、FOXA1、CA XII 和 TRPS1 是敏感的乳腺癌标记物，其中 FOXA1 的敏感性最好，TRPS1 的特异性最高。这些标记物在乳腺癌渗出液中的表达是否能反映患者的生存情况，值得进一步研究。

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来源期刊

Cytopathology 生物-病理学

CiteScore

2.30

自引率

15.40%

发文量

107

审稿时长

6-12 weeks

期刊介绍： The aim of Cytopathology is to publish articles relating to those aspects of cytology which will increase our knowledge and understanding of the aetiology, diagnosis and management of human disease. It contains original articles and critical reviews on all aspects of clinical cytology in its broadest sense, including: gynaecological and non-gynaecological cytology; fine needle aspiration and screening strategy. Cytopathology welcomes papers and articles on: ultrastructural, histochemical and immunocytochemical studies of the cell; quantitative cytology and DNA hybridization as applied to cytological material.