Combined analysis of proteomics and metabolism reveals critical roles of oxidoreductase activity in mushrooms stimulated by wolfberry and sea buckthorn substrates.

IF 4 2区 农林科学 Q2 NUTRITION & DIETETICS
Frontiers in Nutrition Pub Date : 2025-03-18 eCollection Date: 2025-01-01 DOI:10.3389/fnut.2025.1543240
Yuan Zhao, Hongying Li, Youhua Yao, Qing Wei, Tilong Hu, Xia Li, Boxu Zhu, Hailing Ma
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引用次数: 0

Abstract

Background: Cultivating edible fungi, particularly Lentinula edodes, efficiently transforms agroforestry byproducts into valuable products. However, the mechanism of the promotive effects of those substrates was largely unknown. This study used wolfberry (WB) and sea buckthorn (SBK) substrates to investigate mushroom fruiting bodies' physiological, proteomics, and metabolism profiling.

Results: Results show that compared to apple wood (AW), the crude protein and fatty acids were substantially enhanced by both WB and SBK treatment. We identified 1409 and 1190 upregulated and downregulated differentially abundant proteins (DAPs) for the SBK versus AW group and observed 929 overlapped DAPs with upregulation patterns. Of these DAPs, carbohydrates and oxidoreductase activity pathways were significantly enriched. Moreover, the enhanced expression of nine genes by WB and SBK was confirmed by qPCR. Metabolism suggests that 66 differentially abundant metabolites overlapped in the list of two comparison groups (WB versus AW and SBK versus AW).

Conclusion: Collectively, we summarized that both WB and SBK stimulate glucose degradation, enhance the expression of gene-related oxidoreductase activity, and promote protein biosynthesis by coordinating with amino acid metabolism. This study highlights the importance of oxidoreductase activity in promoting nutritional value in mushroom fruiting bodies induced by WB and SBK substrates.

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来源期刊
Frontiers in Nutrition
Frontiers in Nutrition Agricultural and Biological Sciences-Food Science
CiteScore
5.20
自引率
8.00%
发文量
2891
审稿时长
12 weeks
期刊介绍: No subject pertains more to human life than nutrition. The aim of Frontiers in Nutrition is to integrate major scientific disciplines in this vast field in order to address the most relevant and pertinent questions and developments. Our ambition is to create an integrated podium based on original research, clinical trials, and contemporary reviews to build a reputable knowledge forum in the domains of human health, dietary behaviors, agronomy & 21st century food science. Through the recognized open-access Frontiers platform we welcome manuscripts to our dedicated sections relating to different areas in the field of nutrition with a focus on human health. Specialty sections in Frontiers in Nutrition include, for example, Clinical Nutrition, Nutrition & Sustainable Diets, Nutrition and Food Science Technology, Nutrition Methodology, Sport & Exercise Nutrition, Food Chemistry, and Nutritional Immunology. Based on the publication of rigorous scientific research, we thrive to achieve a visible impact on the global nutrition agenda addressing the grand challenges of our time, including obesity, malnutrition, hunger, food waste, sustainability and consumer health.
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