Diagnostic value of serum miRNA-134-3p and miRNA-155-5p for monitoring seizure control in pediatric epilepsy

IF 1.4 4区医学 Q4 CLINICAL NEUROLOGY

Brain & Development Pub Date : 2025-04-03 DOI:10.1016/j.braindev.2025.104357

Yating Yang , Jing Kang , Yiyan Zhang , Yuehao Cai , Qiong Fang , Yukun Huang , Chengyong Huang , Qiaobin Chen , Ying He , Fan Lin

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Abstract

Objective

This study explored the potential of miRNA-134-3p and miRNA-155-5p as biomarkers for assessing seizure severity in children.

Methods

Healthy children and children with epilepsy from Fujian Provincial Hospital (Sept 2022 to July 2023) were grouped into the control group (n = 20), the well-controlled group (n = 22), and the poorly-controlled group (n = 18). Clinical data and serum samples were analyzed using quantitative real-time polymerase chain reaction to detect miRNA levels. Receiver operating characteristic curve analysis evaluated diagnostic potential.

Results

The poorly-controlled group showed increased seizure frequency (P < 0.001), medication dosage (P < 0.001), and intellectual disabilities (P = 0.041). MiRNA-134-3p and miRNA-155-5p levels were higher in epilepsy patients, especially in the poorly-controlled group (P < 0.001). When the critical value of miRNA-134-3p for diagnosing seizure control was 4.336, the specificity was 90.9 % and the sensitivity was 88.9 %. When the diagnostic threshold of miRNA-155-5p was 2.870, the specificity was 63.6 % and the sensitivity was 100 %. The sensitivity, specificity, and accuracy of the combined diagnosis of serum miRNA-134-3p and miRNA-155-5p for seizure control were 88.9 %, 100 %, and 94.45 %, respectively.

Conclusion

The expressions of miRNA-134-3p and miRNA-155-5p were elevated in the serum of children with epilepsy, and were positively correlated with the severity of seizures. The serum miRNA-134-3p has high specificity in diagnosing the degree of seizure control in children, while miRNA-155-5p has high sensitivity. Their combination of the two can improve the specificity of predicting the degree of seizure control in children.

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血清miRNA-134-3p和miRNA-155-5p对监测小儿癫痫发作控制的诊断价值

本研究探讨了miRNA-134-3p和miRNA-155-5p作为评估儿童癫痫发作严重程度的生物标志物的潜力。方法将福建省立医院的健康儿童和癫痫患儿（2022年9月至2023年7月）分为对照组（n = 20）、控制良好组（n = 22）和控制不佳组（n = 18）。采用定量实时聚合酶链反应分析临床数据和血清样本，检测 miRNA 水平。结果控制不佳组的癫痫发作频率（P < 0.001）、用药量（P < 0.001）和智力障碍（P = 0.041）均有所增加。癫痫患者的 miRNA-134-3p 和 miRNA-155-5p 水平较高，尤其是在控制不良组（P < 0.001）。当诊断癫痫发作控制的 miRNA-134-3p 临界值为 4.336 时，特异性为 90.9%，敏感性为 88.9%。当 miRNA-155-5p 的诊断临界值为 2.870 时，特异性为 63.6%，敏感性为 100%。结论癫痫患儿血清中 miRNA-134-3p 和 miRNA-155-5p 的表达量升高，且与癫痫发作的严重程度呈正相关。血清 miRNA-134-3p 在诊断儿童癫痫发作控制程度方面具有高度特异性，而 miRNA-155-5p 则具有高度敏感性。两者结合可提高预测儿童癫痫发作控制程度的特异性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain & Development 医学-临床神经学

CiteScore

3.60

自引率

0.00%

发文量

153

审稿时长

50 days

期刊介绍： Brain and Development (ISSN 0387-7604) is the Official Journal of the Japanese Society of Child Neurology, and is aimed to promote clinical child neurology and developmental neuroscience. The journal is devoted to publishing Review Articles, Full Length Original Papers, Case Reports and Letters to the Editor in the field of Child Neurology and related sciences. Proceedings of meetings, and professional announcements will be published at the Editor''s discretion. Letters concerning articles published in Brain and Development and other relevant issues are also welcome.