Persimmon leaf extract ameliorates hyperlipidemia by modulating lipid genes expression and gut microbiota in high-fat-diet-fed mice.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-03-31 DOI:10.1002/jsfa.14248

Yuanyuan Gao, Jing Liu, Xiaojuan Liu, Yuexin Hao, Zhaofeng Pan, Xiaohua He, Bin Liu, Xuchang Duan

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引用次数: 0

Abstract

Background: Hyperlipidemia is characterized by abnormally elevated blood lipids. Persimmon leaf has multiple pharmacological activities and is valued for its lipid-lowering effect. However, few reports have revealed the hypolipidemic mechanism of persimmon leaf. In this study, compositions from the ethanol extract of persimmon leaf (PLE) were identified by high-resolution liquid chromatography-electrospray ionization-tandem mass spectrometry and high-performance liquid chromatography. The mechanism of PLE against hyperlipidemia induced by high-fat diet in mice was then explored based on lipid gene expression and gut microbiota.

Results: The study demonstrated that 27 compositions from PLE were identified, of which the primary hypolipidemic compositions were astragalin, hyperoside, catechin, chlorogenic acid, and quercetin. Supplementation of PLE could reduce serum lipids, liver injury, lipid accumulation, and inflammation. The analysis of lipid gene expression indicated that PLE downregulated the expression of lipid synthesis genes FAS (fatty acid synthase; P < 0.001), ACC (acetyl coenzyme A carboxylase; P < 0.01), SCD1 (stearyl coenzyme A dehydrogenase 1; P < 0.05) and SREBP-1c (sterol regulatory element binding protein 1c; P < 0.01), while upregulating the expression of lipid degradation genes PPAR-α (peroxide-activated receptor alpha; P < 0.05) and CYP7A1 (cholesterol 7α-hydroxylase; P < 0.05). Simultaneously, PLE greatly recovered the intestinal short-chain fatty acid content, especially butyric acid (P < 0.05), valeric acid (P < 0.01) and isovaleric acid (P < 0.05). Furthermore, 16S rRNA analysis showed that PLE decreased the Firmicutes/Bacteroidetes ratio and increased the abundance of Lactobacillus, Turicibacter, and Dubosiella microbiota, which maintained the homeostasis of intestinal flora.

Conclusion: PLE could prevent lipid metabolism disorders and modulate gut microbiota homeostasis in hyperlipidemic rats. This study provides insights into PLE as a natural active substance for the prevention of hyperlipidemia. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.