Methionine affects the freeze-drying resistance of Lactiplantibacillus plantarum LIP-1 by improving its antioxidant capacity.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-03-30 DOI:10.1002/jsfa.14268

Yijian Zheng, Zichao Chen, Ruixue Wang, Ying Yang, Youxin Yang, Jingjing E, Junguo Wang

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引用次数: 0

Abstract

Background: Lactic acid bacteria is an essential industrial strain, and improving its freeze-drying survival rate is the key challenge to ensuring the activity and stability of bacterial powder. Although medium optimization has been shown to strengthen strain freeze-drying tolerance, the mechanism by which amino acids repair freeze-drying damage in lactic acid bacteria remains unclear. This study investigated the effects of methionine on the freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1 and explored the underlying protective mechanisms.

Results: The study demonstrates that supplementing the medium with 0.06 g/L methionine significantly improved the freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1 (P < 0.05). Further analysis revealed that the strain significantly reduced intracellular reactive oxygen species levels through metabolizing methionine (P < 0.05), decreased the oxidation degree of unsaturated fatty acids in the cell membrane, and reduced cell membrane damage, thereby strengthening the freeze-drying resistance of the strain.

Conclusion: Methionine can enhance the freeze-drying resistance of Lactiplantibacillus plantarum LIP-1 by enhancing antioxidant capacity and maintaining the stability of the subcellular structure. This study provides a specific reference value for improving the freeze-drying survival rate of lactic acid bacteria by modifying the medium conditions. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.