Development of an analytical method for guazatine pesticides in agricultural products using LC-MS/MS.

IF 2.3 3区 农林科学 Q2 CHEMISTRY, APPLIED
So Hee Park, Dogyung Oh, Eun Joo Baek, Tae Woong Na
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Abstract

Guazatine, a non-invasive disinfectant used for seed treatment and post-harvest citrus protection, inhibits bacterial lipid synthesis and consists of about 13 components. While it may occasionally be detected in fruits and vegetables such as citrus, its use as a pesticide has been banned in several countries due to health and environmental concerns. To ensure safe export of domestic produce, a simultaneous method of analysis for guazatine components was developed, focusing on GG, GGG, GGN, and GNG as indicators. Using LC-MS/MS, samples were extracted with a 3% formic acid/acetone mixture and purified with hexane. The method was validated across five agricultural products (mandarin, pepper, potato, brown rice, and soybean), achieving an r2 above 0.995, and detection and quantitation limits of 0.8 and 2.5 µg/L, respectively. Ion suppression was high, with matrix effects ranging from -84.2 to -89.8%. Recovery rates were 70.2-99.6%, with relative standard deviations under 6.8%. Cross-laboratory validation showed recovery rates of 70.3-106.7% and relative standard deviations below 15.0%, meeting CODEX guidelines.

液相色谱-质谱联用技术建立农产品中胍类农药的分析方法。
瓜扎汀是一种用于种子处理和收获后柑橘保护的无创消毒剂,可抑制细菌脂质合成,由约13种成分组成。虽然偶尔会在柑橘等水果和蔬菜中检测到它,但出于健康和环境方面的考虑,一些国家已禁止将其作为杀虫剂使用。为保证国内农产品出口安全,以GG、GGG、GGN、GNG为指标,建立了瓜嗪组分同步分析方法。采用LC-MS/MS,用3%甲酸/丙酮混合物提取样品,用己烷纯化。该方法在5种农产品(柑桔、辣椒、马铃薯、糙米和大豆)中均得到验证,r2 > 0.995,检测限和定量限分别为0.8和2.5µg/L。离子抑制作用强,基质效应范围为-84.2 ~ -89.8%。回收率为70.2 ~ 99.6%,相对标准偏差小于6.8%。交叉实验室验证表明,回收率为70.3-106.7%,相对标准偏差低于15.0%,符合CODEX指南。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.40
自引率
6.90%
发文量
136
审稿时长
3 months
期刊介绍: Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.
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