Xiao Zhang, Jie Li, Xia Wen, Xin-bing Wang, Deng-pan Shen, Li-wei Ding, Jing He
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引用次数: 0
Abstract
Background
Powdery mildew poses a significant threat affecting goji plant cultivation, resulting in significant economic losses, particularly in low UV-B environments. To clarify the molecular mechanism by which UV-B-induced wax synthesis in leaves affects the resistance of goji powdery mildew, the ‘Ganqi II’ was used as the experimental material, natural light (no filtration, FUV-B) was used as the control, and two treatments were compared: polyethylene film filtration (filtering 48.62%, HUV-B) and glass filtration (filtering 98.33%, NUV-B). Differences in leaf wax load, powdery mildew resistance, and gene expression were analyzed under these treatments, and key genes involved in UV-B-induced wax synthesis were identified and validated through qRT-PCR, bioinformatics analysis, subcellular localization, and tobacco overexpression assays.
Results
The results showed that UV-B reduction using polyethylene film and glass decreased the leaf wax load from 90.65 µg/cm2 to 70.53 µg/cm2 and 49.99 µg/cm2, respectively. Concurrently, the incidence of powdery mildew rose from 7.85 to 21.7% and 72.92%, while the disease index increased from 14.21 to 27.23 and 78.40, respectively. Transcriptomic data revealed that 122 differentially expressed genes (DEGs) were significantly enriched in the lipid metabolism pathway, with 26 DEGs specifically associated in the wax synthesis metabolic pathway. Under FUV-B conditions, the expression of LbCYP96A15 was 18.04 times higher than under NUV-B treatment. LbCYP96A15, localized in the endoplasmic reticulum, plays a key role in wax synthesis. Overexpression of LbCYP96A15 in tobacco increased wax load and reduced powdery mildew incidence and disease index compared to the wild type.
Conclusions
UV-B radiation enhances resistance to powdery mildew in goji plants by upregulating the expression of the LbCYP96A15 gene, which promotes leaf wax biosynthesis. LbCYP96A15, a key gene localized in the endoplasmic reticulum, plays a critical role in wax synthesis. Its overexpression significantly increases wax load and reduces the incidence of powdery mildew.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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