Multiomics approach reveals the comprehensive interactions between nutrition and children's gut microbiota, and microbial and host metabolomes.

IF 4.4 2区 医学 Q1 NUTRITION & DIETETICS
Mingyu Zhu, Qi Wang, Yan Yang, Xiaobing Liu, Jiawen Zhang, Guanghao Li, Wenqing Liu, Xuesong Xiang, Juanjuan Chen
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引用次数: 0

Abstract

The gut microbiome can modulate nutrient metabolism to produce many metabolites interacting with the host. However, the intricate interactions among dietary intake, the gut microbiome and metabolites, and host metabolites need to be further explored although some studies have been devoted to it. Here, in a cross-sectional studies, 88 children aged 2-12 years were enrolled from northwestern China. The dietary intake data were collected via a designed food frequency questionnaire to calculate plant-based diet indices (PDIs). Stool and plasma samples were collected for metagenomic and broad-targeted metabolomic analysis. Spearman's rank correlation was used to describe the associations between nutrients/PDIs and the gut microbiota and metabolites. PDI was significantly positively associated with Bilophila wadsworthia, Bacteroides thetaiotaomicron, and Alistipes indistinctus, etc., but was obviously negatively correlated with Roseburia intestinalis, Faecalibacterium prausnitzii, etc. However, these species showed no significant associations with either healthy PDI (hPDI) or unhealthy PDI (uPDI). Interestingly, hPDI was significantly positively related to species, including Ruminococcus bicirculans, and was significantly negatively associated with uPDI, and vice versa. The above correlation trends were also observed between PDIs and predicted gut microbial functional pathways, microbial metabolites and the host metabolome. Notably, the significantly related pathways were focused mainly on substances and energy metabolism. PDI was significantly positively associated with the fecal contents of P-aminobenzoate, chenodeoxycholic acid, 4,6-dihydroxyquinoline, quinoline-4,8-diol, etc., but was significantly negatively associated with those of TMAO, FFA, creatine phosphate, etc. In plasma, PDI was significantly positively associated with sarcosine, ornithine, L-histidine, etc., but was distinctly negatively correlated with FFAs, carnitine C2:0, etc. Strikingly, the healthy plant-based diet index (hPDI) is correlated with increased levels of metabolites related to tryptophan metabolism, whereas the unhealthy PDI (uPDI) is linked to increased levels of metabolites associated with tyrosine and sphingolipid metabolism, which are pathways commonly associated with Western diets. Our studies provide reliable data support and a comprehensive understanding of the effects of dietary intake on the gut microbiome and microbial and host metabolites and lay a foundation for further studies of the diet-gut microbiota-microbial metabolites and host metabolism.

多组学方法揭示了营养与儿童肠道微生物群、微生物和宿主代谢组之间的全面相互作用。
肠道微生物群可以调节营养代谢,产生许多与宿主相互作用的代谢物。然而,饮食摄入与肠道微生物和代谢物以及宿主代谢物之间复杂的相互作用有待进一步探索,尽管已有一些研究。在横断面研究中,88名年龄在2-12岁的儿童从中国西北地区入选。通过设计的食物频率问卷收集膳食摄入数据,计算植物性饮食指数(pdi)。收集粪便和血浆样本进行宏基因组和广泛靶向代谢组学分析。Spearman等级相关被用来描述营养素/ pdi与肠道微生物群和代谢物之间的关系。PDI与华氏杆菌(Bilophila wadsworthia)、拟杆菌(Bacteroides thetaiotaomicron)、模糊拟杆菌(Alistipes disinctus)等呈显著正相关,与肠蔷薇菌(Roseburia intestinal)、prausnitzii粪杆菌(Faecalibacterium prausnitzii)等呈显著负相关。然而,这些物种与健康PDI (hPDI)或不健康PDI (uPDI)均无显著相关性。有趣的是,hPDI与包括双循环鲁米诺球菌在内的物种呈显著正相关,与uPDI呈显著负相关,反之亦然。pdi与预测的肠道微生物功能途径、微生物代谢物和宿主代谢组之间也存在上述相关趋势。值得注意的是,显著相关的途径主要集中在物质和能量代谢。PDI与粪便中对氨基苯甲酸酯、鹅去氧胆酸、4,6-二羟基喹啉、喹啉-4,8-二醇等含量呈显著正相关,与TMAO、FFA、磷酸肌酸等含量呈显著负相关。血浆PDI与肌氨酸、鸟氨酸、l -组氨酸等呈显著正相关,与游离脂肪酸、肉碱C2:0等呈显著负相关。引人注目的是,健康的植物性饮食指数(hPDI)与色氨酸代谢相关的代谢物水平增加相关,而不健康的植物性饮食指数(uPDI)与酪氨酸和鞘脂代谢相关的代谢物水平增加相关,这是通常与西方饮食相关的途径。我们的研究提供了可靠的数据支持,全面了解了膳食摄入对肠道微生物群、微生物和宿主代谢产物的影响,为进一步研究饮食-肠道微生物群-微生物代谢产物和宿主代谢奠定了基础。
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来源期刊
Nutrition Journal
Nutrition Journal NUTRITION & DIETETICS-
CiteScore
9.80
自引率
0.00%
发文量
68
审稿时长
4-8 weeks
期刊介绍: Nutrition Journal publishes surveillance, epidemiologic, and intervention research that sheds light on i) influences (e.g., familial, environmental) on eating patterns; ii) associations between eating patterns and health, and iii) strategies to improve eating patterns among populations. The journal also welcomes manuscripts reporting on the psychometric properties (e.g., validity, reliability) and feasibility of methods (e.g., for assessing dietary intake) for human nutrition research. In addition, study protocols for controlled trials and cohort studies, with an emphasis on methods for assessing dietary exposures and outcomes as well as intervention components, will be considered. Manuscripts that consider eating patterns holistically, as opposed to solely reductionist approaches that focus on specific dietary components in isolation, are encouraged. Also encouraged are papers that take a holistic or systems perspective in attempting to understand possible compensatory and differential effects of nutrition interventions. The journal does not consider animal studies. In addition to the influence of eating patterns for human health, we also invite research providing insights into the environmental sustainability of dietary practices. Again, a holistic perspective is encouraged, for example, through the consideration of how eating patterns might maximize both human and planetary health.
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