A resonance energy transfer electrochemiluminescence sensor based on the synergistic effect of luminol@β-cyclodextrin and Ni-MOF@Pt NPs for the oxytetracycline detection

Abstract

The electrochemiluminescence (ECL) sensor for detecting oxytetracycline (OTC) was constructed by sequentially modifying luminol@β-cyclodextrin (β-CD) and Ni-MOF@Pt NPs on the electrode surface. In this platform, luminol serves as a luminescent reagent, and Pt NPs serve as co-reaction accelerators of the system. β-CD and Ni-MOF are used as carriers to carry luminol and Pt NPs respectively, and the luminescence intensity was further improved by increasing the carrying capacit. In addition, the OTC quenching strategy is adopted, and the constructed sensor is based on the energy transfer of luminol. Under optimal conditions, this sensor exhibited the linear detection range of OTC was found to be between 2.0 × 10⁻¹² M to 2.0 × 10⁻⁷ M, with a detection limit of 6.7 × 10⁻¹³ M (S/N = 3). The sensor showed good high selectivity and ultra-sensitivity, and obtained satisfactory results when detecting honey and milk samples.