Isolation of vanA-Mediated Vancomycin-Resistant Enterococcus faecalis (ST1912/CC116) and Enterococcus faecium (ST80/CC17), optrA-Positive Linezolid-Resistant E. faecalis (ST32, ST1902) from Human Clinical Specimens in Bangladesh.

IF 4.3 2区医学 Q1 INFECTIOUS DISEASES

Antibiotics-Basel Pub Date : 2025-03-04 DOI:10.3390/antibiotics14030261

Sangjukta Roy, Meiji Soe Aung, Shyamal Kumar Paul, Md Nazmul Alam Khan, Syeda Anjuman Nasreen, Muhammad Saiful Hasan, Nazia Haque, Tridip Kanti Barman, Jobyda Khanam, Fardousi Akter Sathi, Shashwata Paul, Mohammad Ibrahim Ali, Nobumichi Kobayashi

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引用次数: 0

Abstract

Background/objectives: Enterococcus is one of the major nosocomial pathogens. The present status of antimicrobial resistance determinants and virulence factors was analyzed for current Enterococcus causing infectious diseases in Bangladesh.

Methods: Clinical isolates of Enterococcus recovered from various specimens in a tertiary care hospital were analyzed. Antimicrobial susceptibility was measured by a broth microdilution test, and resistance genes/virulence factors were detected by uniplex/multiplex PCR, along with sequencing analysis as required. The sequence type (ST) of E. faecalis and E. faecium was identified based on a multilocus sequence typing (MLST) scheme.

Results: For a one-year period, a total of 143 isolates (135 E. faecalis, 7 E. faecium, and 1 E. hirae) were collected. Although all E. faecalis isolates were susceptible to penicillin, high resistance rates were noted against erythromycin (87%) and levofloxacin (62%). High-level resistance to gentamicin was detected in 30% of E. faecalis and 86% of E. faecium. Vancomycin resistance due to vanA was identified in one isolate each of E. faecalis (ST1912, CC116) and E. faecium (ST80, CC17). Three E. faecalis isolates (2.2%) with ST32 or ST1902 were resistant to linezolid, harboring optrA-fexA.

Conclusions: The present study identifies the vancomycin-resistant Enterococcus harboring vanA from humans in Bangladesh and shows the potential spread of optrA in multiple lineages of E. faecalis.

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从孟加拉国人类临床标本中分离出钒介导的万古霉素耐药粪肠球菌（ST1912/CC116）和屎肠球菌（ST80/CC17）， optra阳性耐利奈唑胺粪肠球菌（ST32, ST1902）

背景/目的：肠球菌是主要的医院病原菌之一。分析了孟加拉国目前引起传染病的肠球菌的抗微生物药物耐药性决定因素和毒力因素的现状。方法：对某三级医院临床分离的肠球菌标本进行分析。采用肉汤微量稀释试验检测药敏，采用单/多重PCR检测耐药基因/毒力因子，并根据需要进行测序分析。采用多位点序列分型（MLST）方法鉴定了粪肠杆菌和粪肠杆菌的序列类型（ST）。结果：在1年的时间里，共采集到143株分离株，其中粪肠杆菌135株，粪肠杆菌7株，鼠肠杆菌1株。虽然所有分离的粪肠球菌对青霉素敏感，但对红霉素（87%）和左氧氟沙星（62%）的耐药率较高。30%的粪肠杆菌和86%的粪肠杆菌对庆大霉素有高度耐药性。粪肠杆菌（ST1912, CC116）和粪肠杆菌（ST80, CC17）各有1株对万古霉素耐药。3株（2.2%）携带ST32或ST1902菌株对利奈唑胺耐药，携带optrA-fexA；结论：本研究确定了孟加拉国人类携带vanA的万古霉素耐药肠球菌，并显示optrA可能在多个粪肠球菌谱系中传播。

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来源期刊

Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics

CiteScore

7.30

自引率

14.60%

发文量

1547

审稿时长

11 weeks

期刊介绍： Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.