Background: Highland barley is widely regarded as a premium cereal grain due to its exceptional nutritional profile. This study employed near-infrared spectroscopy technology for the quantitative assessment of five critical parameters in highland barley: total starch, amylose, protein, β-glucan, and total phenols. To optimize spectral data processing, the most effective preprocessing method was identified among six options (standard normal transformation, multivariate scattering correction, normalization (Nor), detrend (DE), first derivative (FD), second derivative (SD)). Furthermore, feature wavelength selection algorithms, including competitive adaptive reweighted sampling (CARS), successive projections algorithm (SPA), uninformative variable elimination, and least angle regression, were utilized to enhance the model's predictive accuracy.
Results: The commendable predictability for total starch was achieved through DE-SPA (Rp2 = 0.913, root mean square error of prediction (RMSEP) = 1.612). For amylose, Nor-CARS exhibited predictive performance (Rp2 = 0.925, RMSEP = 2.049). Protein showcased a creditable result by SD-SPA (Rp2 = 0.876, RMSEP = 0.710). β-Glucan achieved notable predictability through FD-CARS (Rp2 = 0.763, RMSEP = 0.328). Total phenols exhibited remarkable predictability using SD-SPA (Rp2 = 0.946, RMSEP = 0.130).
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The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface.
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