Efficient synthesis of salidroside using mined glycosyltransferase through cascade reaction.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2025-06-01 Epub Date: 2025-03-27 DOI:10.1007/s00449-025-03153-1

Fucheng Zhu, Zixu Yan, Jingli Dai, Juwen Wang, Shiping Huang, Jingbo Ma, Naidong Chen, Yongjun Zang

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引用次数: 0

Abstract

Salidroside has been widely utilized in the food and cosmetics industries. However, the efficient synthesis of salidroside remains a challenge. In this study, a potential uridine diphosphate-dependent glycosyltransferase (UGT) from Bacillus subtilis 168 (named UGT_BS) was identified through evolutionary relationship analysis and molecular docking, with findings subsequently validated by experimental verification. The optimal conversion of UGT_BS for salidroside synthesis reached 98.4% (mol/mol). Additionally, a conversion exceeding 85% (mol/mol) was achieved using the UGT_BS-AtSuSy cascade reaction with tyrosol concentrations ranging from 1 to 10 mM, demonstrating the substrate tolerance of UGT_BS at high concentrations. Kinetic determination and molecular docking confirmed that the strengthened hydrogen bonds and suitable active center conformation between the enzyme and substrate may account for the efficient synthesis of salidroside. Furthermore, 43.5 mM of salidroside was obtained using a fed-batch cascade reaction strategy. The UGT_BS identified in this study shows significant potential for salidroside synthesis, and the strategy employed here serves as a reference for the discovery of related enzymes.

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利用开采的糖基转移酶通过级联反应高效合成红景天苷。

红景天甙在食品和化妆品行业中得到了广泛的应用。然而，红景天苷的高效合成仍然是一个挑战。本研究通过进化关系分析和分子对接，从枯草芽孢杆菌168 （Bacillus subtilis 168）中发现了一个潜在的尿嘧啶二磷酸依赖糖基转移酶（UGT），并通过实验验证了这一发现。UGTBS合成红景天苷的最佳转化率为98.4% （mol/mol）。此外，在酪醇浓度为1 ~ 10 mM的情况下，UGTBS- atsusy级联反应的转化率超过85% (mol/mol)，证明了UGTBS在高浓度下对底物的耐受性。动力学测定和分子对接证实，酶与底物之间的氢键增强和合适的活性中心构象可能是红景天苷高效合成的原因。此外，采用进料批级联反应策略，获得了43.5 mM的红景天苷。本研究鉴定的UGTBS具有较大的红红草苷合成潜力，本文采用的策略可为相关酶的发现提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.