Prolonged Unilateral Renal Ischemia-Reperfusion as a Model for Acute to Chronic Kidney Injury in Female Mice.

Kaitlynn Stowers, Valeria Rudman-Melnick, Qing Ma, Prasad Devarajan
{"title":"Prolonged Unilateral Renal Ischemia-Reperfusion as a Model for Acute to Chronic Kidney Injury in Female Mice.","authors":"Kaitlynn Stowers, Valeria Rudman-Melnick, Qing Ma, Prasad Devarajan","doi":"10.1152/ajprenal.00280.2024","DOIUrl":null,"url":null,"abstract":"<p><p>Acute kidney injury (AKI) is a significant risk factor for developing chronic kidney disease (CKD). Recent studies have highlighted notable gender differences in the susceptibility and expression of both AKI and CKD. The mechanisms underlying these differences remain unclear, and there is a lack of reliable models for studying the AKI-CKD transition in females. In this study, we evaluated various ischemia times in the unilateral renal ischemia-reperfusion injury (UIR) model in female mice to establish a model for studying the AKI-CKD transition. UIR was induced in the left kidneys of male and female C57Bl/6 mice. Kidney pathology and key injury markers were examined 28 days post-UIR.Comparable pathological changes were observed in female mice subjected to 50- and 60-minute ischemia, similar to those in male mice subjected to 30-minute UIR. Protein levels of key injury markers, including Vim-1, Krt8, and Acta2, were significantly increased in female mice subjected to 50- and 60-minute UIR, comparable to male mice subjected to 30-minute UIR, 28 days post-injury. Additionally, an increase in mRNA expression of key kidney injury markers <i>Col1a1</i>, <i>Vim-1</i>, <i>FN</i>, and <i>Sox4</i>, along with a decline in <i>Slc34a1</i> expression, was observed in female mice subjected to 50- and 60-minute UIR, similar to male mice subjected to 30-minute UIR, at 28 days post-injury. Our findings suggest that the optimal ischemia time for inducing CKD changes in female mice is 50-60 minutes, compared to much shorter injury times in male mice.</p>","PeriodicalId":93867,"journal":{"name":"American journal of physiology. Renal physiology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of physiology. Renal physiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1152/ajprenal.00280.2024","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Acute kidney injury (AKI) is a significant risk factor for developing chronic kidney disease (CKD). Recent studies have highlighted notable gender differences in the susceptibility and expression of both AKI and CKD. The mechanisms underlying these differences remain unclear, and there is a lack of reliable models for studying the AKI-CKD transition in females. In this study, we evaluated various ischemia times in the unilateral renal ischemia-reperfusion injury (UIR) model in female mice to establish a model for studying the AKI-CKD transition. UIR was induced in the left kidneys of male and female C57Bl/6 mice. Kidney pathology and key injury markers were examined 28 days post-UIR.Comparable pathological changes were observed in female mice subjected to 50- and 60-minute ischemia, similar to those in male mice subjected to 30-minute UIR. Protein levels of key injury markers, including Vim-1, Krt8, and Acta2, were significantly increased in female mice subjected to 50- and 60-minute UIR, comparable to male mice subjected to 30-minute UIR, 28 days post-injury. Additionally, an increase in mRNA expression of key kidney injury markers Col1a1, Vim-1, FN, and Sox4, along with a decline in Slc34a1 expression, was observed in female mice subjected to 50- and 60-minute UIR, similar to male mice subjected to 30-minute UIR, at 28 days post-injury. Our findings suggest that the optimal ischemia time for inducing CKD changes in female mice is 50-60 minutes, compared to much shorter injury times in male mice.

雌性小鼠急性至慢性肾损伤模型的研究。
急性肾损伤(AKI)是发展为慢性肾脏疾病(CKD)的重要危险因素。最近的研究强调了AKI和CKD在易感性和表达上的显著性别差异。这些差异背后的机制尚不清楚,也缺乏可靠的模型来研究女性AKI-CKD的转变。本研究通过评价雌性小鼠单侧肾缺血再灌注损伤(UIR)模型的不同缺血时间,建立研究AKI-CKD过渡的模型。在雄性和雌性C57Bl/6小鼠左肾诱导UIR。uir后28天检查肾脏病理和主要损伤标志物。在50和60分钟缺血的雌性小鼠中观察到类似的病理变化,与30分钟UIR的雄性小鼠相似。损伤后28天,在50和60分钟紫外线照射的雌性小鼠中,关键损伤标志物(包括Vim-1、Krt8和Acta2)的蛋白质水平显著升高,与30分钟紫外线照射的雄性小鼠相当。此外,在损伤后28天,在50和60分钟UIR的雌性小鼠中,与30分钟UIR的雄性小鼠相似,观察到关键肾损伤标志物Col1a1、Vim-1、FN和Sox4的mRNA表达增加,同时Slc34a1表达下降。我们的研究结果表明,雌性小鼠诱导CKD变化的最佳缺血时间为50-60分钟,而雄性小鼠的损伤时间要短得多。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信