Are vitamin B-12 measurements adequate for evaluating its deficiency in individuals?

IF 1.3 4区医学 Q4 NUTRITION & DIETETICS

Asia Pacific journal of clinical nutrition Pub Date : 2025-04-01 DOI:10.6133/apjcn.202504_34(2).0010

Sedat Özdemir, Selda Demirtaş

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引用次数: 0

Abstract

Background and objectives: Measurement of vitamin B-12 deficiency using different methods may cause diagnostic difficulties. In order to rapidly and safely diagnose vitamin B-12 deficiency, it is important to determine the reference ranges of serum B-12 and its related biomarkers such as homocysteine, holotranscobalamine (holo-TC) and methylmalonic acid (MMA). This study aimed to determine reference interval (RI)s for serum vitamin B-12 and related markers.

Methods and study design: Samples were collected from 404 young-to-middle-aged healthy adults aged 18-65 years. Vitamin B-12, homocysteine, holotranscobalamin, folate were analyzed using the Arcitect i2000 device. Plasma MMA was analyzed by LC/MS. RIs were then evaluated accordingly.

Results: Vitamin B-12, folate, homocysteine, holotranscobalamin and plasma MMA were 139-619 pg/mL, 3.0-14.7 ng/mL, 5.6-18.4 μmol/L, 10.7-101 pmol/L, and 0.01-0.8 μmol/L, respectively. Age group-specific RIs were also generated.

Conclusions: This study revealed that the diagnosis of vitamin B-12 deficiency should not only be based on serum vitamin B-12 levels, but also of folate, homocysteine, holotranscobalamin and MMA levels; all which are related to vitamin B-12 metabolism.

查看原文本刊更多论文

维生素B-12的测量是否足以评估个体的缺乏？

背景和目的：使用不同的方法测量维生素B-12缺乏症可能会导致诊断困难。为了快速、安全诊断维生素B-12缺乏症，确定血清B-12及其相关生物标志物如同型半胱氨酸、全反钴胺（holo-TC）和甲基丙二酸（MMA）的参考范围是很重要的。本研究旨在确定血清维生素B-12及相关指标的参考区间。方法与研究设计：选取404例年龄在18-65岁的健康中青年。维生素B-12、同型半胱氨酸、全反钴胺素、叶酸使用architect i2000设备进行分析。采用LC/MS分析血浆MMA。然后对RIs进行相应的评估。结果：维生素B-12、叶酸、同型半胱氨酸、全钴胺素和血浆MMA分别为139 ~ 619 pg/mL、3.0 ~ 14.7 ng/mL、5.6 ~ 18.4 μmol/L、10.7 ~ 101 pmol/L和0.01 ~ 0.8 μmol/L。还生成了特定年龄组的RIs。结论：维生素B-12缺乏症的诊断不仅应基于血清维生素B-12水平，还应结合叶酸、同型半胱氨酸、全反钴胺素和MMA水平；这些都与维生素B-12的代谢有关

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来源期刊

Asia Pacific journal of clinical nutrition 医学-营养学

CiteScore

2.50

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The aims of the Asia Pacific Journal of Clinical Nutrition (APJCN) are to publish high quality clinical nutrition relevant research findings which can build the capacity of clinical nutritionists in the region and enhance the practice of human nutrition and related disciplines for health promotion and disease prevention. APJCN will publish original research reports, reviews, short communications and case reports. News, book reviews and other items will also be included. The acceptance criteria for all papers are the quality and originality of the research and its significance to our readership. Except where otherwise stated, manuscripts are peer-reviewed by at least two anonymous reviewers and the Editor. The Editorial Board reserves the right to refuse any material for publication and advises that authors should retain copies of submitted manuscripts and correspondence as material cannot be returned. Final acceptance or rejection rests with the Editorial Board