Modification of soluble dietary fiber from quinoa bran and investigation of its anticancer activity in vitro.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-03-24 DOI:10.1002/jsfa.14231

Yunfei Ge, Yu Shi, Chunhong Wei, Dezhi Liu, Longkui Cao, Subramanian Palanisamy, Chongye Fang

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引用次数: 0

Abstract

Background: To investigate the immunological and anticancer actions of quinoa bran soluble dietary fiber (SDF), this study used a simple approach to develop an SDF-zinc complex (SDF-Zn) to enhance the biological activity of SDF. SDF-Zn was co-cultured with NK cells to investigate its ability to activate NK cells. Then, its ability to induce apoptosis in HepG2 human hepatoma cells was investigated using cell proliferation, scratch wound healing assay, flow cytometry, real-time polymerase chain reaction and western blot.

Results: The results revealed that chelating SDF and zinc ions considerably increased the cytotoxicity of NK cells against HeLa cells (48.76% at 150 μg mL^-1) and significantly (P < 0.05) increased the levels of IFN-γ, TNF-α, granzyme-B and NKp44. SDF-Zn was co-cultured with common human cancer cells including AGS, HeLa, HCT116 and HepG2, and the results revealed that SDF-Zn significantly (P < 0.05) inhibited HepG2 cell proliferation. The overproduction of reactive oxygen species increased the expression of apoptotic genes including Bax and caspase-3, and the number of dead cells reached 45.91%. The inhibitory effect of SDF-Zn on HepG2 cells occurred through the MAPK and NF-κB signaling pathways.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.