Comparison of the impacts of cellulase and laccase on fermentation quality, bacterial composition and in vitro degradability of anaerobic cofermentation derived from Sudan grass with mulberry under Lactobacillus plantarum and different lignocellulolytic enzyme inoculation

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2025-03-25 DOI:10.1186/s40538-025-00760-8

Qiang Yu, Ya Su, Yulong Xi, Yuanjiang Rong, Yixi Long, Yixiao Xie, Hong Sun, Rui Dong, Jun Hao, Fuyu Yang, Yulong Zheng

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Abstract

The objective of this study was to evaluate the synergistic and differential effects of inoculation with lignocellulolytic enzymes and ferulic acid esterase (FAE)-producing Lactobacillus plantarum on the fermentation characteristics, bacterial community and in vitro degradability of Sudan grass and mulberry and their mixed silage. Sudan grass and mulberry were mixed at mass ratios of 10:0 (S), 7:3 (S–7), 5:5 (S–5), 3:7 (S–3) and 0:10 (M). With the following treatments were applied: no treatment (CK); L. plantarum alone (LP); L. plantarum, cellulase and xylanase combined (LCX); or L. plantarum, xylanase and laccase combined (LXL). Compared with the control, all the additives (especially the bacterium–enzyme combinations) increased the lactic acid (LA) concentration, water-soluble carbohydrate (WSC) content and relative abundance of Lactobacillus; decreased the pH, ammonia–nitrogen (AN) concentration, coliform count and relative abundance of undesirable bacteria such as Enterobacter; and facilitated lignocellulosic degradation. LCX was more effective in degrading neutral detergent fiber (aNDF) and acid detergent fiber (ADF), decreased the pH, increased the WSC content and simplified the structure of the bacterial network, whereas LXL was better in degrading lignin and enhanced in vitro fermentation efficiency. In addition, LXL improved the silage quality by increasing the acetic acid (AA) concentration and relative abundance of Lactobacillus buchneri. Compared with ensiling alone, mixed ensiling balanced the nutrient composition, reduced the butyric acid (BA) concentration and relative abundance of Enterobacter, increased the relative abundance of Lactobacillus, increased the bacterial network positive correlation ratio and promoted in vitro dry matter (DM) digestibility. Overall, mixed ensiling and bacterium–enzyme inoculation improved fermentation quality.

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.