Transcriptome and metabolome analyses revealed different salt tolerance pathways in leaves and roots of Rosa rugosa Thunb.

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-03-01 DOI:10.1016/j.scienta.2025.114064

Ling Li , Fengqi Zang , Qichao Wu , Yizeng Lu , Shuhan Yu , Yan Ma , Dekui Zang

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引用次数: 0

Abstract

Rosa rugosa Thunb., mainly distributed on the coast in China, has strong salt tolerance, making it a good material to study the salt tolerance mechanism of plants. This study showed that under 200 mM NaCl stress, the activities of superoxide dismutase (SOD) and peroxidase (POD), and soluble sugar content, as well as malondialdehyde (MDA) content in R. rugosa roots and leaves first increased and then decreased. The differentially expressed genes (DEGs) and differentially abundant metabolites (DAMs) enriched in the leaves were significantly more than those in the roots, and they played a dominant role in the response to salt stress. Combined metabolome and transcriptome analyses showed that starch and sucrose metabolism, tryptophan metabolism and purine metabolism played essential roles dealing with salt stress in root. The flavonoid biosynthesis pathway and glycerophospholipid metabolism pathway were significantly enriched in the leaves, which improved antioxidant ability. In addition, abscisic acid (ABA) accumulated in the leaves and roots and was the most important anti-salt hormone in R. rugosa. The study elucidated the molecular mechanism underlying the response of R. rugosa to salt stress and supplied breeding of salt-tolerant R. rugosa with theoretical support.

查看原文本刊更多论文

转录组学和代谢组学分析揭示了蔷薇叶和根不同的耐盐途径。

Rosa rugosa Thunb。主要分布在中国沿海地区，具有较强的耐盐性，是研究植物耐盐机理的良好材料。本研究表明，在200 mM NaCl胁迫下，紫穗槐根系和叶片超氧化物歧化酶（SOD）和过氧化物酶（POD）活性先升高后降低，可溶性糖含量和丙二醛（MDA）含量先升高后降低。叶片中富集的差异表达基因（DEGs）和差异丰富代谢物（DAMs）显著高于根系，在盐胁迫响应中起主导作用。代谢组学和转录组学分析表明，淀粉和蔗糖代谢、色氨酸代谢和嘌呤代谢在根系处理盐胁迫中起重要作用。黄酮类生物合成途径和甘油磷脂代谢途径在叶片中显著富集，提高了抗氧化能力。此外，脱落酸（ABA）在叶和根中积累，是柽柳最重要的抗盐激素。本研究阐明了盐胁迫下黑穗草响应的分子机制，为选育耐盐黑穗草提供了理论支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.