Increase of Lipid Productivity in Coculture of Lipomyces starkeyi and Chlorella saccharophilum on a Picoliter Incubator Array

IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY

ACS food science & technology Pub Date : 2025-02-27 DOI:10.1021/acsfoodscitech.4c0087810.1021/acsfoodscitech.4c00878

Yuma Tanaka, Akihiro Nakamura, Wataru Ogasawara and Ryoji Kurita*,

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Abstract

The coculture of Lipomyces starkeyi and Chlorella saccharophilum can produce lipids more efficiently than the monoculture of L. starkeyi or C. saccharophilum. However, the conventional culture method has problems such as uniformity of light irradiation and the time-consuming growth evaluation of each microorganism. In this study, we cocultured L. starkeyi and C. saccharophilum with a flattened incubator array and demonstrated the live cell imaging of L. starkeyi and C. saccharophilum under a microscope. Our flattened incubator array alleviated cell overlaps in the z-direction and provided the microalgae with uniform light exposure. The initial growth of L. starkeyi and C. saccharophilum during coculture was evaluated by observing their morphology under a microscope. We also demonstrated for the first time that lipid productivity (/day) was increased by coculturing L. starkeyi and C. saccharophilum from a single cell.

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在皮升培养箱阵列上提高斯塔克脂菌与嗜糖小球藻共培养的脂质产量

starkeyomyces starkeyi与糖小球藻（Chlorella saccharophilum）共培养产生脂质的效率高于starkeyi l.s or saccharophilum的单培养。然而，传统的培养方法存在光照不均匀、对每种微生物进行生长评估耗时等问题。在本研究中，我们利用扁平培养阵列共培养L. starkeyi和C. saccharophilum，并在显微镜下展示了L. starkeyi和C. saccharophilum的活细胞成像。我们设计的扁平培养箱阵列减少了细胞在z方向上的重叠，并为微藻提供了均匀的光照。在显微镜下观察双歧杆菌和糖乳杆菌在共培养过程中的初始生长情况。我们还首次证明，通过在单个细胞中培养L. starkeyi和C. saccharophilum，可以提高脂质产量（/天）。

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来源期刊

ACS food science & technology

CiteScore

3.30

自引率

0.00%

发文量