High-Molecular-Weight Polyphenols from Sorghum Bicolor Retain Higher Glycation Inhibition and Cancer Cell Inhibition In vitro Compared to Small-Molecular-Weight Polyphenols after Separation Using Sephadex LH-20

IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY

ACS food science & technology Pub Date : 2025-02-17 DOI:10.1021/acsfoodscitech.4c0085110.1021/acsfoodscitech.4c00851

Ádina L. Santana, Jaymi Peterson, Ramasamy Perumal, Yong Hoon Joo, Seoung-Ho Lee, Kaliramesh Siliveru and Dmitriy Smolensky*,

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引用次数: 0

Abstract

Sorghum contains a diverse array of phenolic compounds, including condensed tannins, phenolic acids, and 3-deoxyanthocyanidins. The differences in bioactivity among these phenolics are not well studied. This work improved existing methods to selectively separate polyphenols based on size using extracts of highly phenolic sorghum varieties. Sephadex LH-20 resin was used to separate large-molecular-weight condensed tannins from oligomeric and monomeric polyphenols. Flavonoid monomers, including 3-deoxyanthocyanidins, were highly concentrated in ethanolic fractions, while the large polyphenol polymers were concentrated in acetone fractions. The bioactivity of separated fractions was evaluated via glycation inhibition and colorectal cancer cell inhibition (HCT116 and RKO). The results demonstrated that high- molecular-weight fractions demonstrated significantly higher (p < 0.05) bioactivity than low- molecular-weight fractions in both assays. This work provides valuable information on the separation of sorghum polyphenols by size and their bioactivity.

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用Sephadex LH-20分离高粱中高分子量多酚后，与小分子量多酚相比，具有更高的糖基化抑制和癌细胞抑制作用

高粱含有多种酚类化合物，包括浓缩单宁、酚酸和3-脱氧花青素。这些酚类物质之间的生物活性差异尚未得到很好的研究。本研究改进了现有的基于高酚性高粱品种提取物大小选择性分离多酚的方法。采用Sephadex LH-20树脂对大分子量缩合单宁与低聚和单体多酚进行分离。类黄酮单体，包括3-脱氧花青素，在乙醇馏分中高度富集，而大的多酚聚合物则集中在丙酮馏分中。通过糖基化抑制和结直肠癌细胞抑制（HCT116和RKO）来评估分离组分的生物活性。结果表明，高分子量组分的(p <；0.05)生物活性均低于低分子量组分。本研究为高粱多酚类化合物的大小分离及其生物活性研究提供了有价值的信息。

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来源期刊

ACS food science & technology

CiteScore

3.30

自引率

0.00%

发文量