Potential of Virus-Mediated RNAi of Insect Genes in Plants to Control Aphids

IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Baozheng Shi, Haifang He, Chenchen Zhao, Caiyan Lei, Jingjing Li, Feng-Ming Yan
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Abstract

Expression of double-stranded RNAs (dsRNAs) in plants is an emerging strategy to efficiently control insects. RNA interference (RNAi)-mediated pest control takes advantage of double-stranded RNA that can suppress the expression of one or more insect genes that encode key proteins. Virus-induced gene silencing (VIGS) is a useful tool for plant expression of dsRNAs to control pests without altering the plant’s genome. Trehalase (TRE) and chitin synthase (CHS) are very important in insects. In this study, we first demonstrated that spraying dsRNAs targeting CHS and TRE increased the mortality rate of the peach aphid Myzus persicae treated with the pathogenic fungus Metarhizium anisopliae. When dsRNAs targeting mpTRE and mpCHS were expressed in plants via VIGS, the expression of mpTRE and mpCHS was reduced in aphids, and their fertility and survival rates were decreased. These results indicate that VIGS-mediated RNA interference is a powerful approach to effectively control aphids, and aphids had a higher mortality rate when M. anisopliae was sprayed.

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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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