Orbital Clinicopathological Differences in Thyroid Eye Disease: An Analysis of Cytokines With Histopathological and Clinical Correlation.

IF 5 2区 医学 Q1 OPHTHALMOLOGY
Yue Li, Jiaqi Tang, Gaojing Jing, Yueyue Li, Rui Ma, Xin Kang, Liyuan Rong, Wenlu Liu, Lan Yao, Xiaohui Lv, Aijun Deng, Wei Wu, Xinji Yang
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Abstract

Purpose: To explore the pathological differences in orbital adipose/connective tissue between active and inactive thyroid eye disease (TED) subjects and their correlations with clinical characteristics.

Methods: Orbital adipose/connective tissue samples from 42 TED subjects (20 active, 22 inactive) were collected during decompression surgery. We analyzed cytokine expression, inflammatory cell infiltration, inherent cell populations, and interstitial changes by Luminex and histopathology. Correlations were analyzed using Pearson and Spearman correlation analyses.

Results: Among the 108 cytokines detected, active TED exhibited elevated platelet endothelial cell adhesion molecule 1 (PECAM-1), interleukin-23 (IL-23), a proliferation-inducing ligand (APRIL), IL-6, C-C motif chemokine ligand 2 (CCL2), β-nerve growth factor (NGF), and lower CCL21 and CCL5. The extent of infiltration by helper T (Th) cells and monocytes was significantly greater in the active group than in the inactive group. Adipocyte density was significantly elevated in active TED, whereas fibrosis was more prominent in inactive TED. Fifteen cytokines were significantly associated with inflammatory cell infiltration, with IL-16 showing the strongest correlations with T cells. Ten cytokines showed significant positive correlations with fibrosis. Four cytokines (IL-6, PECAM-1, IL-23 and transforming growth factor β1), Th cell infiltration and adipocyte density were significantly positively correlated with clinical activity score (CAS). Sixteen cytokines, along with adipocyte density, were significantly positively correlated with disease severity of TED.

Conclusions: The orbital adipose/connective tissues of active and inactive TED subjects showed significant differences in terms of cytokines, inflammatory cells infiltration, inherent cells and interstitium. These pathological changes were correlated with clinical characteristics of TED.

甲状腺眼病眼眶临床病理差异:细胞因子与组织病理学和临床相关性分析。
目的:探讨活动性和非活动性甲状腺眼病(TED)患者眼眶脂肪/结缔组织的病理差异及其与临床特征的相关性。方法:在减压手术中收集42例TED患者(20例活动,22例非活动)的眼眶脂肪/结缔组织样本。我们通过Luminex和组织病理学分析细胞因子表达、炎症细胞浸润、固有细胞群和间质变化。相关性分析采用Pearson和Spearman相关分析。结果:在检测到的108种细胞因子中,活性TED表现出血小板内皮细胞粘附分子1 (PECAM-1)、白细胞介素-23 (IL-23)、增殖诱导配体(APRIL)、IL-6、C-C基序列趋化因子配体2 (CCL2)、β-神经生长因子(NGF)升高,CCL21和CCL5降低。活性组辅助性T (Th)细胞和单核细胞的浸润程度明显大于无活性组。活动期TED中脂肪细胞密度显著升高,而非活动期TED中纤维化更为显著。15种细胞因子与炎症细胞浸润有显著相关性,其中IL-16与T细胞相关性最强。10种细胞因子与纤维化呈显著正相关。4种细胞因子(IL-6、PECAM-1、IL-23和转化生长因子β1)、Th细胞浸润和脂肪细胞密度与临床活动评分(CAS)呈显著正相关。16种细胞因子和脂肪细胞密度与TED疾病严重程度显著正相关。结论:TED活动者与非活动者眼眶脂肪/结缔组织在细胞因子、炎症细胞浸润、固有细胞和间质方面存在显著差异。这些病理改变与TED的临床特征相关。
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来源期刊
CiteScore
6.90
自引率
4.50%
发文量
339
审稿时长
1 months
期刊介绍: Investigative Ophthalmology & Visual Science (IOVS), published as ready online, is a peer-reviewed academic journal of the Association for Research in Vision and Ophthalmology (ARVO). IOVS features original research, mostly pertaining to clinical and laboratory ophthalmology and vision research in general.
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