Harnessing Ogataea parapolymorpha DL-1 alcohol oxidase in Komagataella phaffii for recombinant xylanase expression

IF 4.8 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-03-12 DOI:10.1016/j.fbio.2025.106346

Shoufeng Wang , Wenjie Cong , Mingxuan Wang , Hualan Zhou , Zhenhai Li , Jiguo Wang , Jianguo Zhang

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引用次数: 0

Abstract

Xylanase production attracted attention because it was an important biological macromolecular in food industry, pharmaceutical industry, animal feed, biorefinery industry. Komagataella phaffii, as a famous recombinant protein producer, used alcohol oxidase 1 to oxidize methanol for recombinant xylanase expression. However, low affinity of alcohol oxidase 1 to oxygen was considered as a limitation because of requirement of a large amount oxygen supply, resulting low consumption of methanol. In this study, Ogataea parapolymorpha DL-1 alcohol oxidase (OpAOX) was used to substitute K. phaffii alcohol oxidase 1 for recombinant xylanase expression increment by 26.7°% through increasing specific methanol consumption rate. Furtherly, recombinant xylanase expression increased by 44.5°% after pH adjustment. These results were also verified by recombinant green fluorescent protein expression in this K. phaffii strain with 54.3°% increment. Therefore, K. phaffii harboring OpAOX provided new approach of recombinant xylanse production, and demonstrated a general strategy for other food related by-products production by K. phaffii.

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.