Quality Control Strategies for Differentiation of Kalanchoe Species.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Phytochemical Analysis Pub Date : 2025-03-11 DOI:10.1002/pca.3525

Evelyn A de Andrade, Isadora Machinski, Valter P de Almeida, Sarah A Barr, Wilmer H Perera, Jane Manfron, Flávio L Beltrame, R Thomas Williamson, Wendy K Strangman

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引用次数: 0

Abstract

Kalanchoe species products are commercially available in local markets and by internationally accessible online retailers and may exhibit quality issues because of misidentification from similar common names and anatomical similarities among related species used as feedstock. This study proposes an approach using UPLC-MS/MS^E and HPTLC, coupled with morphoanatomical analysis to establish chemical composition pattern data for five Kalanchoe species. Subsequently, the methods were validated by analyzing commercial products purported to contain 100% Kalanchoe extract. UPLC-MS/MS^E and HPTLC profiles demonstrated that quercetin and kaempferol derivatives were identified as the primary flavonoids in genuine plant extracts. Chemometric analysis showed clear differences in chemical profiles and no similarities between the Kalanchoe plant extracts and commercial products. Different patterns of anticlinal epidermal cell walls and midrib of the leaves and shape and arrangement of the vascular bundles in the petiole were the primary micro-morphological differences observed. Evaluation of commercial samples revealed that products labeled as containing Kalanchoe did not match the pharmacobotanical analysis nor the chemical composition of the species. These methods can be considered important tools for quality control in commercial products derived from Kalanchoe species.

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凤尾莲品种鉴别的质量控制策略。

kalanche物种产品在当地市场和国际在线零售商处都可以买到，但由于用作原料的相关物种之间相似的通用名称和解剖相似性的错误识别，可能会出现质量问题。本研究采用UPLC-MS/MSE和HPTLC相结合的方法，结合形态解剖分析建立了5种kalanche的化学成分模式数据。随后，通过分析声称含有100%凤尾莲提取物的商业产品来验证该方法。UPLC-MS/MSE和HPTLC分析表明，槲皮素和山奈酚衍生物是植物提取物中的主要类黄酮。化学计量学分析表明，卡拉凤梨植物提取物与商业产品的化学成分有明显差异，没有相似之处。叶片背斜表皮细胞壁和中脉形态的不同以及叶柄维管束的形状和排列是主要的微形态差异。对商业样品的评估显示，标记为含有kalanche的产品不符合药理学分析，也不符合该物种的化学成分。这些方法可以被认为是质量控制的重要工具，从卡拉凤蝶种衍生的商业产品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytochemical Analysis 生物-分析化学

CiteScore

6.00

自引率

6.10%

发文量

审稿时长

1.7 months

期刊介绍： Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.