Establishing the electrophysiological feasibility of the rabbit median nerve as an experimental model for carpal tunnel syndrome

IF 2.7 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods Pub Date : 2025-03-09 DOI:10.1016/j.jneumeth.2025.110411

Semra Çınar , Erkut Turan , Ayşe Nur Akkoç

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引用次数: 0

Abstract

Backround

Rabbits are appropriate models for experimental carpal tunnel syndrome (CTS) studies. This study aimed to explore whether the distribution and innervation area of the nerves supplying the thenar muscles of rabbits are similar to those in humans using anatomical, electrophysiological, and histopathological methods.

New method

20 New Zealand rabbits were used to establish reference conduction values for the median and ulnar nerves. Median nerve denervation was performed on the left forelimb of six rabbits, and changes were assessed 33 days post-surgery. Normative data from healthy rabbits were compared with those from denervated rabbits, and comparisons were made between the right and left forelimbs of the denervated rabbits. Thenar and interosseous (2nd, 3rd, and 4th) muscles were used for histopathology. Dissections focused on the thenar muscles and branches of the median and ulnar nerves.

Results

Thenar muscles were 2.5–3 mm in width and 7–8 mm in length), innervated by both the median and ulnar nerves. Normative latency and amplitude values of 1.85 ± 0.30 ms and 7.71 ± 3.50 mV for the median nerve and 1.85 ± 0.19 ms and 6.14 ± 2.50 mV for the ulnar nerve, respectively. Denervation reduced thenar muscle CMAP amplitude (2.51 ± 2.16 mV, p = 0.047) on ulnar nerve stimulation, indicating dual innervation. The median sensory nerve conduction latency (2.55 ± 0.20 ms) was successfully performed for the first time. Histopathological analysis revealed localized atrophy and degenerative changes in the denervated thenar muscles.

Comparison with existing methods

The new method including novel normative data will significantly enhance the evaluation of CTS in experimental rabbit models, paving the way for more accurate and reliable future research.

Conclusion

The innervation patterns of rabbit thenar muscles were similar to those in humans. This data can aid CTS understanding in rabbit models.

查看原文本刊更多论文

建立兔正中神经作为腕管综合征实验模型的电生理可行性。

背景：家兔是实验性腕管综合征（CTS）研究的合适模型。本研究旨在通过解剖、电生理和组织病理学方法探讨兔大鱼际肌神经的分布和支配区域是否与人相似。新方法：20只新西兰兔建立正中神经和尺神经的参考传导值。对6只家兔左前肢进行正中神经去神经支配，观察术后33天的变化。将正常家兔与去神经家兔的规范数据进行比较，并对去神经家兔的左右前肢进行比较。大鱼际和骨间（第二、第三和第四）肌肉用于组织病理学检查。解剖集中在大鱼际肌肉和正中神经和尺神经的分支。结果：大鱼际肌宽2.5 ~ 3mm，长7 ~ 8mm，受正中神经和尺神经支配。正中神经的标准潜伏期和振幅值分别为1.85±0.30 ms和7.71±3.50mV，尺神经为1.85±0.19 ms和6.14±2.50mV。去神经支配使尺神经刺激下鱼际肌CMAP幅值降低（2.51±2.16mV, p=0.047），提示双神经支配。首次成功测定感觉神经正中传导潜伏期（2.55±0.20 ms）。组织病理学分析显示局部萎缩和退行性改变在去神经的鱼际肌肉。与现有方法的比较：新方法包含新的规范数据，将显著增强实验兔模型CTS的评价，为未来更准确、可靠的研究铺平道路。结论：兔大鱼际肌的神经支配模式与人相似。这一数据有助于理解兔CTS模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Neuroscience Methods 医学-神经科学

CiteScore

7.10

自引率

3.30%

发文量

226

审稿时长

52 days

期刊介绍： The Journal of Neuroscience Methods publishes papers that describe new methods that are specifically for neuroscience research conducted in invertebrates, vertebrates or in man. Major methodological improvements or important refinements of established neuroscience methods are also considered for publication. The Journal''s Scope includes all aspects of contemporary neuroscience research, including anatomical, behavioural, biochemical, cellular, computational, molecular, invasive and non-invasive imaging, optogenetic, and physiological research investigations.