Pinocembrin ameliorates non-alcoholic fatty liver disease by activating Nrf2/HO-1 and inhibiting the NF-κB signaling pathway.

IF 2.5 4区 生物学 Q3 CELL BIOLOGY
Weina Chen, Diming Xue, Xia Feng, Yinhang Zhong, Quanqing Li, Weihang Zhang, Guojun Jiang
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引用次数: 0

Abstract

Objectives: The high intake of high-fat diets and changes in sedentary lifestyles have led to an increase in non-alcoholic fatty liver disease (NAFLD). This study aimed to investigate the effect and mechanism of Pinocembrin (Pin) on NAFLD in vivo and in vitro.

Methods: The pharmacodynamics of Pin alone or in combination with ML385 was assessed in high-fat diet (HFD)-mediated NAFLD mice. HepG2 cells were treated with palmitic acid (PA)/oleic acid (OA) (1:2) as an in-vitro model to study the effect of Pin on lipid deposition and oxidative stress. The roles of Pin in glucose and lipid metabolism, inflammation, oxidative stress, and the Nrf2/HO-1/NF-κB pathway were measured.

Results: Pin alleviated lipid deposition, inflammatory response, and oxidative stress in HFD-induced NAFLD mice and PA/OA-induced HepG2 cells. Moreover, ML385 partly attenuated the protection of Pin on inflammatory response and oxidative stress in vivo and in vitro. More importantly, feeding with an HFD significantly decreased the expression of Nrf2 and HO-1, but treatment with Pin increased their expression, accompanied by an increased nuclear transposition of Nrf2.

Conclusion: Taken together, these results indicated that Pin alleviated glucose and lipid metabolism disorders, inflammation, and oxidative stress in NAFLD by activating the Nrf2/HO-1 signaling pathway and restraining the NF-κB pathway.

匹诺曹通过激活Nrf2/HO-1和抑制NF-κB信号通路改善非酒精性脂肪性肝病。
目的:高脂肪饮食的大量摄入和久坐生活方式的改变导致非酒精性脂肪性肝病(NAFLD)的增加。本研究旨在探讨匹诺曹蛋白(Pinocembrin, Pin)对NAFLD的体内外作用及其机制。方法:观察Pin单用或联用ML385对高脂饮食(HFD)介导的NAFLD小鼠的药效学影响。以棕榈酸(PA)/油酸(OA)(1:2)为体外模型,研究Pin对HepG2细胞脂质沉积和氧化应激的影响。测定Pin在糖脂代谢、炎症、氧化应激、Nrf2/HO-1/NF-κB通路中的作用。结果:Pin可减轻hfd诱导的NAFLD小鼠和PA/ oa诱导的HepG2细胞的脂质沉积、炎症反应和氧化应激。此外,ML385在体内和体外部分减弱了Pin对炎症反应和氧化应激的保护作用。更重要的是,用HFD喂养显著降低了Nrf2和HO-1的表达,而用Pin处理则增加了它们的表达,同时Nrf2的核转位增加。结论:综上所述,Pin通过激活Nrf2/HO-1信号通路,抑制NF-κB通路,缓解NAFLD糖脂代谢紊乱、炎症和氧化应激。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Histology and histopathology
Histology and histopathology 生物-病理学
CiteScore
3.90
自引率
0.00%
发文量
232
审稿时长
2 months
期刊介绍: HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.
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