Identification, characterization, and molecular docking of immunomodulatory peptides in Astragalus (Astragalus membranaceus (Fisch.)Bge) seed protein hydrolysates

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-03-08 DOI:10.1016/j.foodchem.2025.143631

Yu Zhao , He Teng , Zhongxian Yu , Shuai Shao , Guangzhe Li , Mingming Yan

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引用次数: 0

Abstract

Developing bioactive peptides from natural plant sources into functional foods and immunomodulators is becoming an attractive approach. In this study, the protein hydrolysates (APH) of Astragalus membranaceus (Fisch.)Bge seed was prepared by alkaline protease digestion method, and components with less than 3KDa were obtained by ultrafiltration (UAPH). The peptide sequence of UAPH was identified by UPLC-MS/MS. silico analysis screened 46 peptides with biological activity. Cell experiments and molecular docking results showed that UAPH can regulate the immune activity of RAW264.7 cells by enhancing cell phagocytic activity and ROS generation, upregulating the expression of TNF -α, IL-6, IL-1β, and TLR4. Peptides DWVSLPG, WVSLPGVP, and FTSIVGNVF are expected to contribute to the immune regulatory activity of UAPH. This study first discovered that the hydrolyzed protein of A. membranaceus seed is a source of immunomodulatory agents, revealing the potential of A. membranaceus seed peptides in developing novel and effective immunomodulatory functional foods.

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黄芪种子蛋白水解物中免疫调节肽的鉴定、表征及分子对接

将天然植物来源的生物活性肽开发成功能性食品和免疫调节剂正成为一种具有吸引力的方法。本研究采用碱性蛋白酶消化法制备了黄芪（Astragalus membranaceus (Fisch.)Bge）种子的蛋白水解物（APH），并通过超滤获得了小于3KDa的成分（UAPH）。通过UPLC-MS/MS鉴定了UAPH的多肽序列。细胞实验和分子对接结果表明，UAPH能通过增强细胞吞噬活性和ROS生成，上调TNF -α、IL-6、IL-1β和TLR4的表达，调节RAW264.7细胞的免疫活性。肽 DWVSLPG、WVSLPGVP 和 FTSIVGNVF 预计将有助于 UAPH 的免疫调节活性。这项研究首次发现了膜衣草种子的水解蛋白是免疫调节剂的来源，揭示了膜衣草种子肽在开发新型、有效的免疫调节功能食品方面的潜力。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.