Optimization and validation of a QuEChERS method for health risk assessment of pesticide residues in tomato, eggplant, and okra samples from Gujarat, India

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2025-03-05 DOI:10.1016/j.jfca.2025.107413

Sivaperumal Perumal , Manisha Arunkumar , Tejal Mehta , Rupal Thasale , Divya Kottadiyil

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引用次数: 0

Abstract

Pesticides, due to their persistence and widespread use, pose significant concerns for human health. This study presents the optimization and validation of a rapid, reliable, and efficient method for the determination of 61 pesticide residues in tomato, eggplant, and okra samples from Gujarat, India, using QuEChERS extraction coupled with UHPLC-q-TOF/MS and GC-MS/MS in Multiple Reaction Monitoring (MRM) mode. SANTE/11312/2021 guidelines was utilized for validation, demonstrating excellent linearity (>0.99), with recovery ranging from 72–124 %. The limits of detection (LOD) ranged from 0.0004 to 0.0065 mg/kg, and the limit of quantifications LOQs ranged from 0.0014 to 0.021 mg/kg, with matrix effects of ± 20 % and RSD values of < 20 %. A total of 25 % of market samples from the western regions of India showed multiple pesticide residues, cypermethrin and pyrethroids, being frequently detected (12.5 %) in eggplant; tebuconazole and monocrotophos detected in 16 (40 %) okra samples, with some exceeding the maximum residue limits (MRLs) prescribed by the EU. Although health risk assessment based on Hazard Quotient (HQ) and Hazard Index (HI) suggested no significant health risks for both adults and adolescents, further monitoring is recommended.

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.