{"title":"Cryo-STEM Tomography for Cell biology Using Thick Lamella.","authors":"Kazuhiro Aoyama, Hiroko Takazaki, Misaki Arie, Hironori Suemune, Shogo Kawai","doi":"10.1093/jmicro/dfaf017","DOIUrl":null,"url":null,"abstract":"<p><p>Electron tomography (ET) is a powerful tool for structural studies in cell biology, but specimen thickness remains a significant limitation. Scanning transmission electron microscopy (STEM) tomography offers advantages in this regard. Recent developments in focused ion beam (FIB) slicing methods for cryo-cell biology have enabled the observation and 3D reconstruction of relatively thick specimens (300-500 nm) using cryo-STEM tomography. Organelles such as mitochondria and the nuclear membrane have been clearly reconstructed, demonstrating the promise of STEM tomography for structural studies in cell biology.</p>","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopy (Oxford, England)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/jmicro/dfaf017","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Electron tomography (ET) is a powerful tool for structural studies in cell biology, but specimen thickness remains a significant limitation. Scanning transmission electron microscopy (STEM) tomography offers advantages in this regard. Recent developments in focused ion beam (FIB) slicing methods for cryo-cell biology have enabled the observation and 3D reconstruction of relatively thick specimens (300-500 nm) using cryo-STEM tomography. Organelles such as mitochondria and the nuclear membrane have been clearly reconstructed, demonstrating the promise of STEM tomography for structural studies in cell biology.
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