Ion-pairing reversed-phase chromatography with inductively coupled plasma-mass spectrometry applied to simultaneous determination of arsenic and selenium species in functional food American ginseng.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-03-04 DOI:10.1002/jsfa.14211

Jianjie Wu, Yuanyuan Zhang, Ping Sheng, Chunjiao He, Hao Liu, Shanyong Gu, Jiaoyang Luo, Meihua Yang

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引用次数: 0

Abstract

Background: The toxicity of heavy metals and harmful elements is highly dependent on their chemical species. However, research on the simultaneous analysis of inorganic and organic species in complex matrices remains limited. This study presents a rapid and sensitive method for the concurrent determination of arsenic and selenium species using reversed-phase chromatography with inductively coupled plasma-mass spectrometry. Enzyme-assisted extraction was employed to enhance the efficiency of target analyte recovery.

Results: By utilizing ion-pairing reagents and a mobile phase consisting of 20 mmol L^-1 citric acid with 2.0% methanol at pH 4.0, six arsenic species and five selenium species were simultaneously analyzed in kinetic energy discrimination mode within 10 min. The limits of detection for arsenic and selenium species ranged from 0.058 to 0.6 μg kg^-1 and 0.083 to 0.283 μg kg^-1, respectively. The precision of the spiked American ginseng samples ranged from 1.07% to 11.08%, with recovery rates between 72.13% and 107.2%. The method successfully identified and quantified 11 elemental species.

Conclusion: The analysis of real American ginseng samples revealed that the predominant arsenic species were inorganic arsenic(V), while the primary selenium species was organic selenomethionine. This study provides a valuable and efficient method for the simultaneous monitoring of elemental species in functional foods, contributing to their safety assessment and quality control. © 2025 Society of Chemical Industry.

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背景：重金属和有害元素的毒性在很大程度上取决于其化学种类。然而，在复杂基质中同时分析无机和有机物种的研究仍然有限。本研究采用反相色谱-电感耦合等离子体质谱法同时测定砷和硒。该方法采用酶辅助萃取，以提高目标分析物的回收率：利用离子配对试剂和由 20 mmol L-1 柠檬酸与 2.0% 甲醇（pH 值为 4.0）组成的流动相，在 10 分钟内以动能分辨模式同时分析了 6 种砷和 5 种硒。砷和硒的检出限分别为 0.058 至 0.6 μg kg-1 和 0.083 至 0.283 μg kg-1。加标西洋参样品的精密度为1.07%～11.08%,回收率为72.13%～107.2%。该方法成功地鉴定和定量了11种元素：结论：对真实西洋参样品的分析表明，砷的主要种类是无机砷（V），而硒的主要种类是有机硒蛋氨酸。这项研究为同时监测功能食品中的元素种类提供了一种宝贵而有效的方法，有助于对功能食品进行安全评估和质量控制。© 2025 化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.