Engineering UDP-sugar synthesis pathways for exopolysaccharide biosynthesis in Streptococcus thermophilus AR333.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-03-03 DOI:10.1002/jsfa.14206

Yizhou Fan, Junkang Shi, Xin Song, Guangqiang Wang, Yongjun Xia, Lianzhong Ai, Zhiqiang Xiong

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引用次数: 0

Abstract

Background: Exopolysaccharide (EPS) produced by Streptococcus thermophilus can significantly improve the viscosity, texture and taste of dairy products, which have broad application potential in the food industry. However, EPS production is typically low (< 100 mg L^-1) in S. thermophilus, making it difficult to meet industrialization requirements. The utilization of metabolic engineering for genetic modification of S. thermophilus is an efficient approach to enhance EPS biosynthesis.

Results: To our knowledge, there is lack of systematic investigation on engineering UDP-sugar synthetic pathways for EPS production in S. thermophilus. In the present study, the biosynthetic genes of EPS precursors UDP-glucose and UDP-galactose were regulated for improving EPS production in S. thermophilus AR333. Compared with the control, engineered strains by single overexpression of eight EPS precursor genes increased EPS production by 7-31%, respectively. Among of them, overexpressing glk encoding glucokinase and galE1 encoding UDP-galactose-4-epimase led to 275.37 and 288.65 mg L^-1 of EPS production, respectively. Moreover, co-overexpression of lacZ encoding β-galactosidase and galE1 achieved a remarkable 49% increase in the EPS production (329.51 mg L^-1). Transcriptional analysis further suggested that enhanced EPS synthesis in engineered strain can attributed to the upregulation of precursor genes and clusters of EPS genes.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.